Sequences from confirmation amplicon


Confirmation sequences for line 126B02 and hit At5g49620 are shown.
Line ID 126B02
Locus At5g49620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20138589
Primers used for PCR 07hg - 8474, show primer details
   
Sequence Name 38-K112479-0022-126-B02C-07hg
GenBank Accession LN503034 [GenBank]
Sequencing primer gene specific primer, 07hg: 5'-ATTTAAGCTGTTTTGCATGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112479-0022-126-B02C-07hg
GTTCTTCCTGGTAAATATTGTGCAATCTTAGACCATCTGTCCTCATAAGAAGAAGAAAAC
TTGTGATCAAATTCATTACTAGTACAAATAAACCAATCTTTCAAACTCTTGTTAACAGAA
AATAAAATGTATTGATGACTAAAGATGTGAACTAATTCGTGTACAATAATATTTAGCCAT
CTTTGTGATCAAAATCTTGTTAGTAGAAAATAAAGTGTATAAATGACCGTAATTAAAAAA
CGTACCTATTGCCCCAACGTGTGTGAAGTTCAAGAATCAAGAGTTGTTCTTCGAGGGTTA
TGTTTCCACGGCGCACATCTGGTCGGAGATAGTTCAGCCACCGAAGTCTGCAGCTTTTTC
CGGTCCTTTTGAGTTCTGTTTTATATTGGAAGTTACTATTATTTAATTAGACATCATCGT
GTACACGTATATTGTATGATGATTTCATACGCAAGAAACTCATGATAATGATCATATATA
TTTGGTAAAGTTATCAAATTTACTCTAGAAATTATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 08-K112478-0022-126-B02C-8409
GenBank Accession LN489797 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112478-0022-126-B02C-8409
GCATTTACATTGATATAATTTCTAGAGTAAATTTGATAACTTTACCAAATATATATGATC
ATTATCATGAGTTTCTTGCGTATGAAATCATCATACAATATACGTGTACACGATGATGTC
TAATTAAATAATAGTAACTTCCAATATAAAACAGAACTCAAAAGGACCGGAAAAAGCTGC
AGACTTCGGTGGCTGAACTATCTCCGACCAGATGTGCGCCGTGGAAACATAACCCTCGAA
GAACAACTCTTGATTCTTGAACTTCACACACGTTGGGGCAATAGGTACGTTTTTTAATTA
CGGTCATTTATACACTTTATTTTCTACTAACAAGATTTTGATCACAAAGATGGCTAAATA
TTATTGTACACGAATTAGTTCACATCTTTAGTCATCAATACATTTTATTTTCTGTTAACA
AGAGTTTGAAAGATTGGTTTATTTGTACTAGTAATGAATTTGATCACAAGTTTTCTTCTT
CTTGTGAGGACAGATGGTCTAAGATTGCACAATATTTACCAGGAAGAACGGATAACGAGA
TCAAAAACTATTGGAGAACACGTGTTCAAAAGCATGCAAA

Last Updated on 06.12.2018 16:11