Genotyping and primer details


Genotyping and primer details for 126B02 - At5g49620
Line ID 126B02
Locus At5g49620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20138589 of line 126B02 Chr5:20138589
   
Insertion allele amplicon 8474 - 07hg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07hg: 5'-ATTTAAGCTGTTTTGCATGCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20138012
   
wt amplicon (over InsPos) 13ig - 07hg
wt amplicon start Chr5:20138012
wt amplicon end Chr5:20138958
wt amplicon size 947 bp
Locus specific primer 07hg: 5'-ATTTAAGCTGTTTTGCATGCTTTT-3'
2nd locus specific primer 13ig: 5'-CAAGAAATGGATTTTGCCTTGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11