Sequences from confirmation amplicon


Confirmation sequences for line 127F06 and hit At4g05040 are shown.
Line ID 127F06
Locus At4g05040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2580970
Primers used for PCR ML31 - 8474, show primer details
   
Sequence Name 81-K100817-0022-127-F06C-8409
GenBank Accession LN514027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100817-0022-127-F06C-8409
CATTTACATTGAATATTGATCGGTCTAACAGGTTGCATACTCCTTTATAAAACCCTATGG
ATGCTGCAAAGGATAGACAAGTCCTTCCTTCTTCATCTCGCTCGTCCTCAAGACTTGGAT
ATTCGTTAAGAATAACATCAAGGACACCTGCTCAAACAATTGGGAACAACAATTAGCATA
ACGCAGCTGGTTTTACATAAGAAATTTGAACAGACTAAATGCCAAAGCGAGAGATAAACC
TATACTTCTGGCATTCAAAGCAACATGTACAAGATGTTTTCTCCCTTCCAACTTTGAATC
TAAGTTAGAGTTCCTCCCTTCAAGGTCATTGTTACCTGCGGTTTTCAAAATTTCTTTCAC
AAGAGTCACATCTCCAGCTTCTACTGCCATGTACAAGGAAGATATTCCCTCATTATTTTC
AAGAAAAGAAGCATTCTGGTTCTCGTTCACCAGAGAGGCAGCCATCTCCATATAGCGTCC
TTCAATGGCCAAGTGCAGAGCAGTATTTCCATATTTGTCTCTGAGAACATATGGATTTAG
TCTCTCTCTATCTTCTTCAGCCAGTCTATCTGAAAAAAATGTTA
   
Sequence Name 81-K100829-0022-127-F06C-ML31
GenBank Accession LN514029 [GenBank]
Sequencing primer gene specific primer, ML31: 5'-TCAAATTATCGGATCTTTTCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100829-0022-127-F06C-ML31
GGAAGTTTTTGCGGACTGAGTGATGGGGACAAGAGTGTCTGGAGATGTTGAAAGGCGTTG
GAACGCCAATGGCATGTCTCAAGAGCGATAGAGGAGATTCAGTTCTTCATCTTGCTGCTA
GATGGGGTCATCTAGAACTAGTGAAGAACATTATCTCTGAATGCCCATGCCTTGTATTGG
AGTTAAACTTCAAGGATCAGCTTCCGCTTCATGTGGCAGCTCATGCCGGTCATTCGGCTA
TTGTTGAGGCTCTTGTTGCGTCGGTAACATTTTTTTCAGATAGACTGGCTGAAGAAGATA
GAGAGAGACTAAATCCATATGTTCTCAGAGACAAATATGGAAATACTGCTCTGCACTTGG
CCATTGAAGGACGCTATATGGAGATGGCTGCCTCTCTGGTGAACGAGAACCAGAATGCTT
CTTTTCTTGAAAATAATGAGGGAATATCTTCCTTGTACATGGCAGTAGAAGCTGGAGATG
TGACTCTNGTGAAAGAAATTTTGAAAACCGCAGGTTACAATGACCTTGA

Last Updated on 06.12.2018 16:11