Sequences from confirmation amplicon


Confirmation sequences for line 128H07 and hit At1g33140 are shown.
Line ID 128H07
Locus At1g33140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12024040
Primers used for PCR 22xn - 8474, show primer details
   
Sequence Name 17-K119249-0022-128-H07C-22xn
GenBank Accession LN494084 [GenBank]
Sequencing primer gene specific primer, 22xn: 5'-TAGGAGCTAAGACGATGATATGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119249-0022-128-H07C-22xn
GCTAACATAGATACCATCAAGAAACTTCCTGATATCCTTCTTCTTCACGTGACATTTCTG
CACAACAATCACAACATAAAAGATTACAACAAAGACTTGAACACGTAAATCAATCACTGA
AATGATCCTTTTGATTTCACTATGCAAAAATGTGATCCTAAACTCAAACATCAACCAAAA
AAACACTTTCATACACAATGCTGAAAAGCTTAAGAAGCCAAACAATAGGCAAAATTGGCA
AAGTTTACTTAGCGAGTAATCTAATCGTCTATTAGAGACCTATAGAGATAATACAAATCG
ATTTTCACTTCATTTATGCAAAATCTGATTCCTAGAACTGAACCGTGTCACCTATCTAAC
ACAATCTCTCAACCTTTTAGTCCCAAGAAAATGCAAATTTGGCTACAAATCTACATGGAT
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTC
   
Sequence Name 01-K119248-0022-128-H07C-8409
GenBank Accession LN486802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119248-0022-128-H07C-8409
ATCCTGATCCATGTAGATTTGTAGCCAAATTTGCATTTTCTTGGGACTAAAAGGTTGAGA
GATTGTGTTAGATAGGTGACACGGTTCAGTTCTAGGAATCAGATTTTGCATAAATGAAGT
GAAAATCGATTTGTATTATCTCTATAGGTCTCTAATAGACGATTAGATTACTCGCTAAGT
AAACTTTGCCAATTTTGCCTATTGTTTGGCTTCTTAAGCTTTTCAGCATTGTGTATGAAA
GTGTTTTTTTGGTTGATGTTTGAGTTTAGGATCACATTTTTGCATAGTGAAATCAAAAGG
ATCATTTCAGTGATTGATTTACGTGTTCAAGTCTTTGTTGTAATCTTTTATGTTGTGATT
GTTGTGCAGAAATGTCACGTGAAGAAGAAGGATATCAGGAAGTTTCTTGATGGTATCTAT
GTTAGCGAGAAAAGCAAGATCGTAGAGGAAGAATGAATGCCCATATCATCGTCTAAGCTC
CT
Primers used for PCR 19xn - 8474, show primer details
   
Sequence Name 18-K119249-0022-128-H07C-19xn
GenBank Accession LN494535 [GenBank]
Sequencing primer gene specific primer, 19xn: 5'-CAAAACTTAAAACAAGCCACGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119249-0022-128-H07C-19xn
TACTAAAGAGGTTGTCTAAAAGCATTTTCTGTGAAAAATGGCTGGCCCTGATCCCTCGTA
ATCTGCCTTTGATATCCACACCAAACATCAGTACTTTGTGTGAATAACTTGAATCATCAT
CACACGGTTAAAGAGTTTACGGTACCTGTTGGAATGTGCTAAGAGAAGCCAAGATGGATC
CTCCAATATTCTTCTCAGGTGGTGCAACCACCTGCTGCTAGGTGCCAACCCCGTGATCTC
TTTGGTTATCCGATCAGCAATTCCTGGAAACATGGTTGATCCGCCACTGAGCACAATGTT
AACATACTAGTCCTTTCTGATGTCGAAATCACATTTCATGATTAAATTATAAGTTGTCTC
ATGAATCCTCGGTGCTTCCAATATGATAAACGATGGTTGAAAGAGGACTTCTGGAAAGCA
GAATCATTCTGCACTAATATTGATCACCTGACCATCAGGGCGTCTCGTAGCTTTTCTCAA
ATTGCAAAGCTCGCTTTCGTTGTCTCAAGTTATCTTTATGTTTGATCACAGCGTTGCGAG
AAATTGATTTTAGTTTAGTGACTGAAGTATCAATTTTGAATGCACAACCCACAATGCCAA
ATCCAGAACAATCCCAAATTCACACGATATGATTTAATTAAGCAAAGGTGAAAATGATAG
CTCGTCTCCAAAACTTGTGCATAAGTAAATAAAATCTCGAACACAAAAAGGGAACAAGGG
ATAGCCAGAGTTTTGCAACACCGGTGGATTTGGTCCATAANACGAAACTAACATTCAAAA
GACAAGAAATAGGAGCTAAGACGATGATATGGGCATTCATTCTTCCTCTACGATCTTGCT
TTTCTCGCTAACATAGATACCATCAAGAAACTTCCTGATATCCTTCTTTCTCACGTGACA
TT
   
Sequence Name 02-K119248-0022-128-H07C-oriL
GenBank Accession LN487258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119248-0022-128-H07C-oriL
CATTTCATTGAATTATCCTGATCCTGTAGATTTGTAGCCAAATTTGCATTTTCTTGGGAC
TAAAAGGTTGAGAGATTGTGTTAGATAGGTGACACGGTTCAGTTCTAGGAATCAGATTTT
GCATAAATGAAGTGAAAATCGATTTGTATTATCTCTATAGGTCTCTAATAGACGATTAGA
TTACTCGCTAAGTAAACTTTGCCAATTTTGCCTATTGTTTGGCTTCTTAAGCTTTTCAGC
ATTGTGTATGAAAGTGTTTTTTTGGTTGATGTTTGAGTTTAGGATCACATTTTTGCATAG
TGAAATCAAAAGGATCATTTCAGTGATTGATTTACGTGTTCAAGTCTTTGTTGTAATCTT
TTATGTTGTGATTGTTGTGCAGAAATGTCACGTGAAGAAGAAGGATATCAGGAAGTTTCT
TGATGGTATCTATGTTAGCGAGAAAAGCAAGATCGTAGAGGAAGAATGAATGCCCATATC
ATCGTCTTAGCTCCTATTTCTTGTCTTTTGAATGTTAGTTTCGTTTTATGGACCAAATCC
ACCGGTGTTGCAAAACTCTGGCTATCCCTTGTTCCCTTTTTGTGTTCGAGATTTTATTTA
CTTATGCACAAGTTTTGGAGACGAGCTATCATTTTCACCTTTGCTTAATTAAATCATATC
GTGTGAATTTGGGATTGTTCTGGATTTGGCATTGTGGGTTGTGCATTCAAAATTGATACT
TCAGTCACTAAACTAAAATCAATTTCTCGCAACGCTGTGATCAAACATAAAGATAACTTG
AGACAACGAAAGCGAGCTTTGCAATTTGAGAAAAGCTACGAGACGCCCTGATGGTCAGGT
GATCAATATTAGTGCAGAATGATTCTGCTTTCCAGAAGTCCTNCTTTCACCATCGTTTAT
CATATTGGAAGCACCGAGGATTCATGAGACAACTTATAATTTTATCATGAAATGTGATTT
Primers used for PCR 48xo - 8474, show primer details
   
Sequence Name 21-K119356-0022-128-H07C-48xo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 48xo: 5'-CAATATTATTAACGGGTCATGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K119356-0022-128-H07C-48xo
TTTTTGGTTTTCTTATGGGCACGATTTTAGTGGGCTGAATTTTGATAAAGGCCCACATAT
GAAAGATATTTAGGGTTTTTTCTCTTGATACCTTTTAAAACGCCGTCGAGAAATCTCCCT
CTAGGGTTTCTTTGATCAAACCAGAGAGCCGTCAGGGAGAGCAAAACAAAGATCGGAGAA
GATGAAGACGATTCTTTCTTCCGAAACGATGGACATCCCCGACAGTGTTACCATCAAGGT
TCACGCTAAAGTGATCGAAGTCGAAGGACCTCGCGGGAAGCTTGTTCGCGATTTCAAGCA
TCTCAACCTCGATTTCCAGCTGATCAAGGATCCAGAGACTGGAAAGAAGAAGCTTAAGAT
CGATTCGTGGTTTGGAACACGCAAAACCAGCGCCTCCATCAGAACCGCTCTTAGCCACGT
CGATAACTTGATCTCCGGTGTTACCAGAGGTTTCCGTTACAAGATGAGGTTCGTGTACGC
CCATTTTCCCATCAACGCCTCCATCGGCGGTGACGGAAAGTCTATCGAGATCCGTAACTT
CCTTGGCGAGAAGAAGGTTTGTTTTATCTTCTTCATCTCTGATTTGATTCTCATAGATTC
GTGTAGATCTCGGAATGAAATAGACTGACACTAGATTCAACTTTATGATTGGACGATGAT
GATTGATTTCTGAGTTTGCGAGCACTGAACTTACATAGAAATGTGTTTATTGGAATAATA
GGTGAGGAAGGTAGAGATGTTGGATGGTGTAACCATTGTTCGATCTGAGAAGGTGAAGGA
TGAGATTGTTCTTGACGGTNACGACATCGAGCTTGTTTCAAGGTCATGCGCTTTGATCAA
CCAGTACTCTCTCTAATAGCAGGATATATTCAATTGTAAATGGCTTCCTGTCCGGGAAAT
CTACATGGATCA
   
Sequence Name 01-K119355-0022-128-H07C-8409
GenBank Accession LN486804 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119355-0022-128-H07C-8409
CATTTCATTGAATATATCCTGCTAGTTAGAGAGAGTACCTGGTTGATCAAAGCGCATGAC
CTTGAAACAAGCTCGATGTCGTTACCGTCAAGAACAATCTCATCCTTCACCTTCTCAGAT
CGAACAATGGTTACACCATCCAACATCTCTACCTTCCTCACCTATTATTCCAATAAACAC
ATTTCTATGTAAGTTCAGTGCTCGCAAACTCAGAAATCAATCATCATCGTCCAATCATAA
AGTTGAATCTAGTGTCAGTCTATTTCATTCCGAGATCTACACGAATCTATGAGAATCAAA
TCAGAGATGAAGAAGATAAAACAAACCTTCTTCTCGCCAAGGAAGTTACGGATCTCGATA
GACTTTCCGTCACCGCCGATGGAGGCGTTGATGGGAAAATGGGCGTACACGAACCTCATC
TTGTAACGGAAACCTCTGGTAACACCGGAGATCAAGTTATCGACGTGGCTAAGAGCGGTT
CTGATGGAGGCGCTGGTTTTGCGTGTTCCAAACCACGAATCGATCTTAAGCTTCTTCTTT
CCAGTCTCTGGATCCTTGATCAGCTGGAAATCGAGGTTGAGATGCTTGAAATCGCGAACA
AGCTTCCCGCGAGGTCCTTCGACTTCGATCACTTTAGCGTGAACCTTGATGGTAACACTG
TCGGGGATGTCCATCGTTTCGGAAGAAAGAATCGTCTTCATCTTCTCCGATCTTTGTTTT
GCTCTCCCTGACGGCTCTCTGGTTTGATCAAAGAAACCCTAGAGGGAGATTTCTCGACGG
CGTTNTAAAAGGTATCAAGAGAAAAAACCCTAAATATCTNTCATATGTGGGCCTTTATCA
AATTTCAGCCCACTAAAATCGTGCCCATAAAGA

Last Updated on 06.12.2018 16:11