Genotyping and primer details


Genotyping and primer details for 128H07 - At1g33140
Line ID 128H07
Locus At1g33140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12024040 of line 128H07 Chr1:12024040
   
Insertion allele amplicon 8474 - 19xn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19xn: 5'-CAAAACTTAAAACAAGCCACGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12025390
   
Insertion allele amplicon 8474 - 22xn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22xn: 5'-TAGGAGCTAAGACGATGATATGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12024529
   
Insertion allele amplicon 8474 - 48xo
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 48xo: 5'-CAATATTATTAACGGGTCATGGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12022416
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11