Sequences from confirmation amplicon


Confirmation sequences for line 129C05 and hit At3g48860 are shown.
Line ID 129C05
Locus At3g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18118827
Primers used for PCR DE37 - 8474, show primer details
   
Sequence Name 50-K105356-0022-129-C05C-DE37
GenBank Accession LN507165 [GenBank]
Sequencing primer gene specific primer, DE37: 5'-TTCCTAATCTAATGCCGCCTAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105356-0022-129-C05C-DE37
TCCTCCCTTGATCCTCAACTCGGAGCAGAGATAAAAGGTGATAACACTTTGAAGTTCTTA
AAAATTGTGCTTCTTATTATAGTTCTCAAATCTTGGTAGTCAAGGGAACGTGTGACTTAG
TGGAATATGTCAAAACCAGTTTGGTTGCTCTTTGGAGTTGGTGTATAATATGATTTTTCG
TTGACTAAATATTTATCCTCAAAGCCTAATAATAGAGGTTGCTATAAAGGTTCATGAGAA
ATGGGATCCTTGCTCCAAGAATTAGAGATCCCTGCTGGTTTTTGGTTGTGCGTCTTGCGT
TTGATTTCGGATATTTGATGTTTTTAGGTTCTTTGCTGATGTACCATCAGTAAATTCAAA
AGAAAAGGGTGATCAGCGGGAAGCTTCAGCTCTTCGTGATGAAGTATGTGATTGTTTCTT
TTGAACCTTTTGCGGTATCTCGTCAATTTAGTTATTGTAAATTTTAGTCAAATTAGAATT
TGTGTATGAGTCATAAGAGTTGACTTGGGGCTGAAAAAGTCAACTCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTC
   
Sequence Name 34-K105357-0022-129-C05C-8409
GenBank Accession LN501413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105357-0022-129-C05C-8409
TTTCGCCCCAGTCACTCTTATGACTCATACACAAATTCTAATTTGACTAAAATTTACAAT
AACTAAATTGACGAGATACCGCAAAAGGTTCAAAAGAAACAATCACATACTTCATCACGA
AGAGCTGAAGCTTCCCGCTGATCACCCTTTTCTTTTGAATTTACTGATGGTACATCAGCA
AAGAACCTAAAAACATCAAATATCCGAAATCAAACGCAAGACGCACAACCAAAAACCAGC
AGGGATCTCTAATTCTTGGAGCAAGGATCCCATTTCTCATGAACCTTTATAGCAACCTCT
ATTATTAGGCTTTGAGGATAAATATTTAGTCAACGAAAAATCATATTATACACCAACTCC
AAAGAGCAACCAAACTGGTTTTGACATATTCCACTAAGTCACACGTTCCCTTGACTACCA
AGATTTGAGAACTATAATAAGAAGCACAATTTTTAAGAACTTCAAAATGTTATCACCTTT
TATCTCTGCTCCGAGTTGGAGGATCAAGTGGAGGAATAGAAACCGGAGTTTTCACCGACA
CCCTACTAGGCGGCATT
Primers used for PCR 23wp - 8474, show primer details
   
Sequence Name 43-K118837-0022-129-C05C-23wp
GenBank Accession LN505012 [GenBank]
Sequencing primer gene specific primer, 23wp: 5'-AGGTACAAATTTTCTGCACAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118837-0022-129-C05C-23wp
TAAGAAGACTCATCTGAGCAGCAAATAGATTGAGGGTATTAACCTCTCTTTGACGCAATG
CTGCTTCTTTCCTATACAAAGACATATTCAACATGAGCGATTAAAAACTACCACCTTGGG
TTTCTGAAAAAGAGAGCAAGAAAAGAGAAGCTAAAGAAGGCACAGGCTCCCACCTGCTTA
ACAATTTCGCCTCCAAGGAAACTCCTTCTCCAAGAGAGGCAACCTTTTGGAACCAAAAAT
AAAAACAAATGAAACCTTGAACAGATGCCAATACATTAGAAATATATGATTTAAAGTAAA
TTTAAACAACGAGGTGGGATGCTGTTTCAAAAAGATAACAACAAGATATACAAAGAGATT
TAAACAAGTGGAGTATTTTTTTCAGTCAGAATTTCAAGACGCAACATAAGTGAAAAATGG
ACTACTCAAGACAACCAGTTCAAAAACCTGTTTCTCGAGCTCCTTAGCCCTAGCTTCTGC
TTCCACCCTTTTTTCTTCCGCACGACGAAGCTGTAATCAAGAATAAATTTTATGAACAAC
AGAAGTAAACAGCTTTATTACATAATATAAAATTCAATCGCAGAGATCAAACCTTCTCCA
GTACATTCTCATTCTCCTCTTGTCCCGGACAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K118836-0022-129-C05C-8409
GenBank Accession LN494978 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118836-0022-129-C05C-8409
CATTTCATTGAATATATCCTGTCCGGGACAAGAGGAGAATGAGAATGTACTGGAGAAGGT
TTGATCTCTGCGATTGAATTTTATATTATGTAATAAAGCTGTTTACTTCTGTTGTTCATA
AAATTTATTCTTGATTACAGCTTCGTCGTGCGGAAGAAAAAAGGGTGGAAGCAGAAGCTA
GGGCTAAGGAGCTCGAGAAACAGGTTTTTGAACTGGTTGTCTTGAGTAGTCCATTTTTCA
CTTATGTTGCGTCTTGAAATTCTGACTGAAAAAAATACTCCACTTGTTTAAATCTCTTTG
TATATCTTGTTGTTATCTTTTTGAAACAGCATCCCACCTCGTTGTTTAAATTTACTTTAA
ATCATATATTTCTAATGTATTGGCATCTGTTCAAGGTTTCATTTGTTTTTATTTTTGGTT
CCAAAAGGTTGCCTCTCTTGGAGAAGGAGTTTCCTTGGAGGCGAAATTGTTAAGCAGGTG
GGAGCCTGTGCCTTCTTTAGCTTCTCTTTTCTTGCTCTCTTTTTCAGAAACCAAGGTGGT
AGTTTTTAATCGCTCATGTTGAATATGTCTTTGTATAGGAAAGAAGCAGCATTGCGTCAA
AGAGAGGTTAATACCCTCAATCTATTTTGCTGCTCAGATGTAGTCTTCTTTTAATCGAGT
CATTATGTAAAACATACAATTTAAGCTGTGCAG
Primers used for PCR AP33 - 8474, show primer details
   
Sequence Name 44-K118837-0022-129-C05C-AP33
GenBank Accession LN505348 [GenBank]
Sequencing primer gene specific primer, AP33: 5'-GTCAGAATTTCAAGACGCAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118837-0022-129-C05C-AP33
TCAAAACCTGTTTCTCGAGCTCCTTAGCCCTAGCTTCTGGCTTCCACCCTTTTTTCTTCC
GCACGACGAAGCTGTAATCAAGAATAAATTTTATGAACAACAGAAGTAAACAGCTTTATT
ACATAATATAAAATTCAATCGCAGAGATCAAACCTTCTCCAGTACATTCTCATTCTCCTC
TTGTCCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 20-K118836-0022-129-C05C-oriL
GenBank Accession LN495434 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118836-0022-129-C05C-oriL
TCATTGAATATATCCTGTCCGGGACAAGAGGAGAATGAGAATGTACTGGAGAAGGTTTGA
TCTCTGCGATTGAATTTTATATTATGTAATAAAGCTGTTTACTTCTGTTGTTCATAAAAT
TTATTCTTGATTACAGCTTCGTCGTGCGGAAGAAAAAAGGGTGGAAGCAGAAGCTAGGGC
TAAGGAGCTCGAGAAACAGGTTTTTGAACTGGTTGTCTTGAGTAGTCCATTTTTCACTTA
TGTTGCGTCTTGAAATTCTGA

Last Updated on 06.12.2018 16:11