Sequences from confirmation amplicon


Confirmation sequences for line 129D02 and hit At1g54130 are shown.
Line ID 129D02
Locus At1g54130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20211553
Primers used for PCR DN44 - 8474, show primer details
   
Sequence Name 13-K101828-0022-129-D02C-8409
GenBank Accession LN491978 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101828-0022-129-D02C-8409
TACATTGAATATATCCTGGTAACAGTCCTCCGATGAGGATTTCACGCGATCGGAACTTAG
ATGGTGGATCTGCTCTTCGTGTAGGATCTAGCAGATTGTTCAATGGTTTCGTCAGGAAAG
CGATAGGTTCATGTGTTGATTACGATACAGACTCTGTTCTTGTCGATGAGCAGCTACCGT
TTACCATGGACGATGGTTTCGAAGGAGAAAGAAGACAACCATATGCTAGGGATTTGCTTA
GACGTGCTCAATTGAAACACAAAATCTTCGAAGATGAATCTGTGATCAAAGCATTTTACG
AAGCTGAGAAAGCTCATAGGGGACAGGTGTGTGGAAGAAGAAGTAAATCTCAAAAACTTG
TCAATAATTTTGGTTTTGATTTTGCTAATTGATGTGTTTGAATCAGATGAGAGCAACTGG
TGATCCATACCTGCAACATTGTGTGGAGACTGCAATGTTGTTGGCTGACATTGGAGCTAA
TTCGACTGTTGNGGTAGCT
   
Sequence Name 57-K101831-0022-129-D02C-DN44
GenBank Accession LN509370 [GenBank]
Sequencing primer gene specific primer, DN44: 5'-CCAGCTACAACAACAGTCGAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K101831-0022-129-D02C-DN44
TGCAGGTATGGATCACCAGNTTGCTCTCATCTGATTCAAACACATCAATTAGCAAAATCA
AAACCAAAATTATTGACAAGTTTTTGAGATTTACTTCTTCTTCCACACACCTGTCCCCTA
TGAGCTTTCTCAGCTTCGTAAAATGCTTTGATCACAGATTCATCTTCGAAGATTTTGTGT
TTCAATTGAGCACGTCTAAGCAAATCCCTAGCATATGGTTGTCTTCTTTCTCCTTCGAAA
CCATCGTCCATGGTAAACGGTAGCTGCTCATCGACAAGAACAGAGTCTGTATCGTAATCA
ACACATGAACCTATCGCTTTCCTGACGAAACCATTGAACAATCTGCTAGATCCTACACGA
AGAGCAGATCCACCATCTAAGTTCCGATCGCGTGAAATCCTCATCGGAGGACTGTTACCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GAAGATGTCCGCA

Last Updated on 06.12.2018 16:11