Sequences from confirmation amplicon


Confirmation sequences for line 129E06 and hit At2g38330 are shown.
Line ID 129E06
Locus At2g38330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16065799
Primers used for PCR 10sj - 8474, show primer details
   
Sequence Name 39-K116478-0022-129-E06C-10sj
GenBank Accession LN503485 [GenBank]
Sequencing primer gene specific primer, 10sj: 5'-ACTTTGATTTGAGGAGAGAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116478-0022-129-E06C-10sj
TGCAATCAAGTATCTGAAGAAACGTTGACAAGTGAGAAACATAAGAAGCCACAAATTAAG
ATAGATATGCTTTACTAGAAAACGAGTCTATTACCACATTTTTGGACATACTCAGAAATC
ACAGTAGCAGCCGCAGCACCAGAGATACCAAAACCAAGGACAAATATCAGGATTGGATCC
AATACCGCATTAAGTACATTCCCCGCAACTGTTGATATAGAGTTCACCATATTAGAAACT
GCCTTATAGGATCAGTTAGCAATGAGTTTTATCAATATCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K116477-0022-129-E06C-8409
GenBank Accession LN492667 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116477-0022-129-E06C-8409
CCATTTACATTGAATATATCCTGATATTGATAAAACTCATTGCTAACTGATCCTATAAGG
CAGTTTCTAATATGGTGAACTCTATATCAACAGTTGCGGGGAATGTACTTAATGCGGTAT
TGGATCCAATCCTGATATTTGTCCTTGGTTTTGGTATCTCTGGTGCTGCGGCTGCTACTG
TGATTTCTGAGTATGTCCAAAAATGTGGTAATAGACTCGTTTTCTAGTAAAGCATATCTA
TCTTAATTTGTGGCTTCTTATGTTTCTCACTTGTCAACGTTTCTTCAGATACTTGATTGC
ATTTATTCTTCTATGGAAGTTGAATGAGAATGTAGTTTTGCTCTCTCCTCAAATCAAAGT
AGCAGC

Last Updated on 06.12.2018 16:11