Sequences from confirmation amplicon


Confirmation sequences for line 129G07 and hit At3g43860 are shown.
Line ID 129G07
Locus At3g43860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15708130
Primers used for PCR HP84 - 8474, show primer details
   
Sequence Name 13-K033539-022-129-G07C-8409
GenBank Accession LN491933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K033539-022-129-G07C-8409
TCACATTTTCACCAAAATCAACAAAATAGTCTTTGTTTTAAACATTAACAAAAGAGTTTT
AAACAATATAGTATTTATGTATAAAAAGATGCATAGATTGATAAGCATACCAATTTGGCT
TTGTTGAGAAGCCGGCGTGCATATTTGTGGTCGACATGGCGGAAAACGATGGAGGAAGCG
GCGAATGCAGCAGCGGCTTCCGCCGCGATCTCGGTTCCTGGAACTTTATCGCTTATCTCC
AACACCGTCCTTGGTGTCTTCATGTTCTCAGGTCTCGCCCAGCACTGGTGGTCCGCGTTT
GGATCACCAACCTATTTGATCATATTCATAGGCACAACCATTTTAAGTAATAAAACGGTA
CGTTCATGTTTCTAGCAT
   
Sequence Name 13-K033539-022-129-G07C-HP84
GenBank Accession LN491934 [GenBank]
Sequencing primer gene specific primer, HP84: 5'-TATGCTAGAAACATGAACGTACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K033539-022-129-G07C-HP84
TATGATATGATCAATAGGTTGGTGATCCAAACGCGGACCACCAGTGCTGGGCGAGACCTG
AGAACATGAAGACACCAAGGACGGTGTTGGAGATAAGCGATAAAGTTCCAGGAACCGAGA
TCGCGGCGGAAGCCGCTGCTGCATTCGCCGCTTCCTCCATCGTTTTCCGCCATGTCGACC
ACAAATATGCACGCCGGCTTCTCAACAAAGCCAAATTGGTATGCTTATCAATCTATGCAT
CTTTTTATACATAAATACTATATTGTTTAAAACTCTTTTGTTAATGTTTAAAACAAAGAC
TATTTTGTTGATTTTTGGTAGAAAATGTGATATATTTTTTTTAGTAAAATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11