Sequences from confirmation amplicon


Confirmation sequences for line 129H03 and hit At3g09070 are shown.
Line ID 129H03
Locus At3g09070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2768930
Primers used for PCR 07vo - 3144, show primer details
   
Sequence Name 76-K117997-0022-129-H03C-07vo
GenBank Accession LN513459 [GenBank]
Sequencing primer gene specific primer, 07vo: 5'-CGGCGATAGGAAAGTGAATAGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K117997-0022-129-H03C-07vo
GGGGAGTGGAGCATTTTAGGACTTATTTACGGAAGAGTGTTAACAAGTATGAGGAAGAGG
AGGAGGAGGAAGAGGATAGGTATAGGAGATTAAACGGTGGAATGGTGGAGAGATCGTTAT
CGGAGTCATGGCCGGAGCTGAGGAATGGAGGAGGAGGAGGAGGAGGGCCGAGGATGGTGA
GGAGTAATAGCGATGTGAGCTGGAGGAGTTCCGGAGGTGGATCATCGAGGAAAGTCAACG
GATTGGATAGGAGGAATAAGAGTTCCAGGTCCTCACCTCAGAATGGGGAAAACGGAATGT
TGAAGTTTTACTTGCCACATATGAAAGCTAGTCGGAGAATGAGCGGTCCCGGAGGAGCAC
GAGGTGGCGGCGGCGGATGGTGGGTAACCGAAAGCGGAAAACGACCCTCTGATCCCCGGG
GACCCAGTCCTCTCCACATGAGCTGAACATCCTCATAAAGAGGAACGGTCTCC
   
Sequence Name 36-K117996-0022-129-H03C-35St
GenBank Accession LN502393 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117996-0022-129-H03C-35St
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTACCCACCCTCCGCCGCCGC
CACCTCCTGCTCCTCCTGTACCGCTCATTCTCCGACTAGCTTTCATATGTGGCAAGTAAA
ACTTCAACATTCCGTTTTCCCCATTCTTAGGTGAGTACCTTGAACTCTTATTCCTCCTAT
CCAATCCGTTGACTTTCCTCGCTGATCCACCTCCGGAACTCCTCCAGCTCACATTGCTAT
TACTCCTCACCATCCTCGGCCCTCCTCCTCCTCCTCCTCCATTCCTCAGCTCCGGCCATG
ACTCCGATAACGATCTCTCCACCATTCCACCGTTTAATCTCCTATACCTATCCTCTTCCT
CCTCCTCCTCTTCCTCATACTTGTTAACACTCTTCCTGTAAATAAGTCCTAAAATGCTCC
ACTTCCCCCACCGTCTCGACTTCTTGTTATTATCGTTACTATTCACTTTCCTATCGCCG

Last Updated on 06.12.2018 16:11