Sequences from confirmation amplicon


Confirmation sequences for line 130G06 and hit At1g33840 are shown.
Line ID 130G06
Locus At1g33840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12285099
Primers used for PCR CD66 - 3144, show primer details
   
Sequence Name 42-K104270-0022-130-G06C-CD66
GenBank Accession LN504449 [GenBank]
Sequencing primer gene specific primer, CD66: 5'-CACAAGGAACATAACAGAGGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104270-0022-130-G06C-CD66
TATTGTTTTTTTTTCTCCTTTTTAATATAAATATCTAAGTTTAAAAACTATTAGAATTTT
TAAAAGAAAAATGTATATAATAAGTTTATCCCCGTAAACTTTTCTCTTTTAAAAGTCAAC
ATATTCAAGGCAAATGTTTACTTTATAAATTCTAAAAACCTTACACTTTAACTTCACGGC
ATAACCAGACCCAAAAAAAAAAGCATATCAAAACAAAACTGAAAAAAAGTACCCATATTT
GGAGGAAAAAAAAAAAAAGGCACCACCCCTATAAGTACCGTTACCCACAAGGCACGGGCC
CTTCGGCTCCCCCCCCGCCTCCAAAACCCGGAGGGATAGGGAATCCAAAGTACGGCTCTC
TGCCCCCGGTAAATTTGGCAATCGTACGGAAGGGGTTAAAAATAACGGACGGAAACTTCG
TGATAACAAAGGCGAAAGGAAACTTGTTGTTCAGGGGGAAGGATCACCAAATTTACAATG
GGAAAACCCGGATAGTTTAAACCAAAGGCGGGAAACAACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAAAGAAAGGGTCTTGCAAAGGATAGGGG
GATTGTGCGTCATCCCTTACGTCGGGGGAGATATCAC
   
Sequence Name 58-K104271-0022-130-G06C-3144
GenBank Accession LN509695 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K104271-0022-130-G06C-3144
TTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTACCAATTGTAAAT
TTGCTGATCCTTCACCTTGAACAACAAGTTTCCTTCCGCATTCGTTATCACGAAGTTACC
GTCCGTTATCTTCAACACCTTCCGTACGATCGCCAAATCTACAGGGTGCAGAGAGCAGTA
CTTTGGATCCACTATCACTCCGGCTTTTGGAGGCGGCGGAGGAGCCGAAGGGCCAGTGCC
TTGTGGGTAACGGTACTCATAGGGCTGCTGCATTCTTTTCTTTTTCCTCCAAATATGGAT
ACTCTCTCTCAGTTTTGTTTTGATATGCTTTTTTTTTTGGGTCTGGTTATGCCGTGAAGT
TAAAGTGTAAGGTTCTTAGAATTTATAAAGTAAACATCTGCCTCGAATATGTTGACTTTT
AAAAGAGAAAAGTCTACGGGGATAAACTTATTATATACATTTTTCTTTTAAAAATTCTAA
TAGTTTTTAAACTAAGATATTTATATATAAAAAGGAGAAAAAAAAAACAATATGTAGGGA
AATAAAAAAACCCTAACCACTGGTTCCTCTGTTATGTCCCTGGGG

Last Updated on 06.12.2018 16:11