Sequences from confirmation amplicon


Confirmation sequences for line 131G04 and hit At1g20925 are shown.
Line ID 131G04
Locus At1g20925 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7292326
Primers used for PCR PW04 - 8474, show primer details
   
Sequence Name 15-K024601-022-131-G04C-8409
GenBank Accession LN492799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K024601-022-131-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACATCGAATCGCGGTATTTTTGTGAGTCTGAAG
AACGAACCAAGATTCATCGCTGGGGGAACGACATACTGAAGCAGAAGAACAAACTGGTAA
AGCGGTTCGGATGTAACCAAACCCAAGTAATGCGCTCCTCGTACTATGAAAACTCCTAAT
ATTGGCAGCAACAAGTAACGCACAACCACCACACCCATTATGACTGACTTGTTTATTCCT
GAACCTCTCAGACCTGTGATTATTATAAGAGAGAGAACAGAGATTTAAGGTTTTGTCTTA
GGAAAAAAAGTTTCTGCAGCCATTTGTTATTGTTTATCTACCATTGAGAAGGTTTCCTCC
AACAATCAGAGTCAGAACAGGAATCGCCCCGTCCCTTCAAGAAAATTAAGCTACTCAAGT
AAGCATTGTTAATTATCACTGACAAGTGAGATTTTTAATATGTTGTAGAATACCCCAATA
GAGATACTGAGTCCTCAATCACTCGAAGTGGAGCTGTGTTACCGACTAGTAGCTTCCGTA
GTAA
   
Sequence Name 16-K024601-022-131-G04C-PW04
GenBank Accession LN493268 [GenBank]
Sequencing primer gene specific primer, PW04: 5'-CAAACAAAGAGTGTGCTCTGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K024601-022-131-G04C-PW04
AGAGAAAATCAATCTACGAACAATATTTGCTCCTTCAACTATTGCAGCGGTTTGTTTCAA
TGTTCCCTTGAACTAAACGACTGCTATCTAGCTTCTGTATGTTCTTACTAGTTAAGCAAC
TAATCCTCTTTCTCTTCAGCTTATCGCGCTTGCGGTTGGGTTAAATCCTTTACTACGGAA
GCTACTAGTCGGTAACACAGCTCCACTTCGAGTGATTGAGGACTCAGTATCTCTATTGGG
GTATTCTACAACATATTAAAAATCTCACTTGTCAGTGATAATTAACAATGCTTACTTGAG
TAGCTTAATTTTCTTGAAGGGACGGGGCGATTCCTGTTCTGACTCTGATTGTTGGAGGAA
ACCTTCTCAATGGTAGATAAACAATAACAAATGGCTGCAGAAACTTTTTTTCCTAAGACA
AAACCTTAAATCTCTGTTCTCTCTCTTATAATAATCACAGGTCTGAGAGGTTCAGGAATA
AACAAGTCAGTCATAATGGGTGTGGTGGTTGTGCGTTACTTGTTGCTGCCAATATTAGGA
GTTTTCATAGTACGAGGAGCGCATTACTTTGGGTTTGGTTACATCCGAACCGCTNTACCA
GTTTGTTCTTCTGCTTCAGTATGTCGTTCCCCCAGCGATGAAATCTGGTTCGTTCTTCAG
ACTCACAAAAATACCGCGATTCGATGTAAATGGCTTCATGTCCGGGAAATCT

Last Updated on 06.12.2018 16:11