Sequences from confirmation amplicon


Confirmation sequences for line 131H12 and hit At5g46050 are shown.
Line ID 131H12
Locus At5g46050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18676928
Primers used for PCR CN20 - 8474, show primer details
   
Sequence Name 64-K030838-022-131-H12C-8409
GenBank Accession LN511118 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K030838-022-131-H12C-8409
ATTGAATAATCCTGTACATTTGTAACTGTTATTTATACATGCATTAAATAGAAGTGTAAA
AGAAAACAATCGAATCTTAAACCGACATTTACATGTACGTACGTAATGGATAAGTCTTTA
GCTAATTAGTTTATTTTATGTTTGTTCCGATCCACAATTTTTCTCGTGTGTACAAAAACT
TTCTAGGATTTTCGATCGAGACAAAAAGACTTAATGCTGTAATAATGTTCTTTCAAAGTC
AACTGTTGGTCAAAGTACACATATAGTTCCAAAACAAATACACATTTTTCTCGAAGAAAA
GTATTGCACGTCTTCA
   
Sequence Name 65-K030838-022-131-H12C-CN20
GenBank Accession LN511318 [GenBank]
Sequencing primer gene specific primer, CN20: 5'-ATTGAAGCGTGCAATACTTTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K030838-022-131-H12C-CN20
TGTGTACTTTGACCACAGTTGACTTTGAAAGAACATTATTACAGCATTAAGTCTTTTTGT
CTCGATCGAAAATCCTAGAAAGTTTTTGTACACACGAGAAAAATTGTGGATCGGAACAAA
CATAAAATAAACTAATTAGCTAAAGACTTATCCATTACGTACGTACATGTAAATGTCGGT
TTAAGATTCGATTGTTTTCTTTTACACTTCTATTTAATGCATGTATAAATAACAGTTACA
AATGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11