Sequences from confirmation amplicon


Confirmation sequences for line 132B04 and hit At5g13650 are shown.
Line ID 132B04
Locus At5g13650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4397732
Primers used for PCR NC07 - 8474, show primer details
   
Sequence Name 09-K101217-0022-132-B04C-8409
GenBank Accession LN490104 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K101217-0022-132-B04C-8409
AATCCTCTCCACCAATAAAATCCTTTAAGCTCCCCTCTCTCTCCCATTCTCTTCTCCCTT
TTCTCTGTTTCGCGGCGGAGAAAATTGAAATGGAGCTCAGCTTGAGCACTTCCTCCGCTT
CTCCTGCGGTTTTGAGGAGACAAGCTTCTCCTCTGCTTCACAAGCAGCAAGTGCTCGGCG
TCAGCTTTGCTTCCGCGCTCAAACCTGGAGGAGGAGCTCTTCGATTTCCTTCCCGCCGTC
CGCTTCCTCGTCCCATCACTTGCTCCGCCTCTCCATCAACTGCCGAACCAGCCTCCGGTT
ATTTTTTACTCTCTCTTATCTCTTCCGATAGTTTTATTACTGGAAATTGATTTCGTTTTT
GTCGAGTTAAGTCAGTTTGAAATAAGAAAACTAGTCAATCAAAGTTCTAAGAATTATCAC
ACGTAGGTTAGTTTCAATATATATATGTATTTTGGTGGTTGATTGGTTCTCTAAATAAAA
TCACTCTTGTTATGTTATGCCAGTAGAGGTGAAGAAGAAGCAGTTGGATAGGAGGGATAA
CGTGAGGAATATCGCAATCGTTGCACATGTTGATCATGGCAAAACTACTTTGGTTGATTC
TATGCTTAGGC
   
Sequence Name 09-K101223-0022-132-B04C-NC07
GenBank Accession LN490105 [GenBank]
Sequencing primer gene specific primer, NC07: 5'-ACCTTAGCTTGCCTAAGCATAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K101223-0022-132-B04C-NC07
ATCACATGTGCAACGATTGCGATATTCCTCACGTTATCCCTCCTATCCGACTGCTTCTTC
TTCACCTCTACTGGCATAACATAACAAGAGTGATTTTATTTAGAGAACCAATCAACCACC
AAAATACATATATATATTGAAACTAACCTACGTGTGATAATTCTTAGAACTTTGATTGAC
TAGTTTTCTTATTTCAAACTGACTTAACTCGACAAAAACGAAATCAATTTCCAGTAATAA
AACTATCGGAAGAGATAAGAGAGAGTAAAAAATAACCGGAGGCTGGTTCGGCAGTTGATG
GAGAGGCGGAGCAAGTGATGGGACGAGGAAGCGGACGGCGGGAAGGAAATCGAAGAGCTC
CTCCTCCAGGTTTGAGCGCGGAAGCAAAGCTGACGCCAAGCACTTGCTGCTTGTGAAGCA
GAGGAGAAGCTTGTCTCCTCAAAACCGCAGGAGAAGCGGAGGAAGTGCTCAAGCTGAGCT
CCATTTCAATTTTCTCCGCCGCGAAACAGAGAAAAGGGAGAAGAGAATGGGAGAGAGAGG
GGAGCTTAAAGGATTTTATTTGGTTGGAGAGGATTTCATTAAGCGATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAGAAATGTAG

Last Updated on 06.12.2018 16:11