Sequences from confirmation amplicon


Confirmation sequences for line 132C07 and hit At5g42230 are shown.
Line ID 132C07
Locus At5g42230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16882269
Primers used for PCR CD44 - 8474, show primer details
   
Sequence Name 04-K104268-0022-132-C07C-CD44
GenBank Accession LN487900 [GenBank]
Sequencing primer gene specific primer, CD44: 5'-TAGAAGATGGCTATTGTGTCGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K104268-0022-132-C07C-CD44
TTGCCGAGGGTATCCGGAAACGATTTGGTTGTGAGGCTTCCTGGTCAACCAAAGGTTGTA
TTCAGACAGTACGCAGGGTATGTTGATCTCGACTTGAATGCTGGCCGTAGCCTTTTCTAC
TACTTCGTGGAAGCTGAAAAACACCCTGACACAAAACCGTTAACCTTGTGGCTCAATGGA
GGTTCGTATTTAGTTGCTCTGCTTCCTAACTAATTAGCATTTGAGTATATCACAAATCAC
AAATTTGTGAAATGAATAAGTTGAGGTTTGTTTTATAGGTCCAGGTTGTTCTTCTGTTGG
TGGAGGAGCTTTCACTGAGCTAGGTCCATTTTACCCCACAGGATATGGGCGTGGTCTTCG
CATTAACTCCATGTCGTGGAACAAAGGTCTATTTCTTATAATATTATGCAAAGAATGAGA
GAAAACAGAACTTTGATCAGCTGACGTTATTGTCTATGGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATATAGATATCCGCACGGT
   
Sequence Name 20-K104269-0022-132-C07C-8409
GenBank Accession LN495193 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K104269-0022-132-C07C-8409
CTTTACAATAGACAATAACGTCAGCTGATCAAGTTCTGTTTTCTCTCATTCTTTGCATAA
TATTATAAGAAATAGACCTTTGTTCCACGACATGGAGTTAATGCGAAGACCACGCCCATA
TCCTGTGGGGTAAAATGGACCTAGCTCAGTGAAAGCTCCTCCACCAACAGAAGAACAACC
TGGACCTATAAAACAAACCTCAACTTATTCATTTCACAAATTTGTGATTTGTGATATACT
CAAATGCTAATTAGTTAGGAAGCAGAGCAACTAAATACGAACCTCCATTGAGCCACAAGG
TTAACGGTTTTGTGTCAGGGTGTTTTTCAGCTTCCACGAAGTAGTAGAAAAGGCTACGGC
CAGCATTCAAGTCGAGATCAACATACCCTGCGTACTGTCTGAATACAACCTTTGGTTGAC
CAGGAAGCCTCACAACCAAATCTGTTTCCGGATACCCTCGTGCAAACACCTGCACTGTCA
CCATCACCATGGCCACATCGCGCAACGACAC

Last Updated on 06.12.2018 16:11