Sequences from confirmation amplicon


Confirmation sequences for line 132F02 and hit At3g13460 are shown.
Line ID 132F02
Locus At3g13460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4385612
Primers used for PCR 03gz - 8474, show primer details
   
Sequence Name 24-K112307-0022-132-F02C-03gz
GenBank Accession LN497056 [GenBank]
Sequencing primer gene specific primer, 03gz: 5'-CTGTCCCATCTTTCTGTTTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112307-0022-132-F02C-03gz
CTGTGCTCTTTTACTTTGTTTATGATATTGTTCTTTATACTCCAGTTTTTGTCTTTCAGG
TCAATGCAAGTGGACAATTTGTTGGTCTTGCTGAAATGACAGGACCAGTTGATTTCAACA
CAAATGTGGAGTACTGGCAGCAAGATAAGTGGACCGGCTCTTTCCCCCTCAAGTGGCATA
TTGTGAAGGATGTGCCAAACAGTTTACTGAAGCATATTACTTTAGAGAACAATGAGAACA
AACCTGTTACCAACAGCAGAGACACACAAGAGGTAAATATTTGTGACATCTTTTGGCTTG
TTTTACTGATTACTCCACGAGCGTTTTTGTTTTCTTGTGCCTAACTTTCTTTGTTTGGAT
CATATTAGGTTAAGTTGGAGCAAGGTTTGAAGATTGTGAAAATTTTCAAGGAGCATAGCA
GTCCGCAATGGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACGGTTATT
   
Sequence Name 08-K112306-0022-132-F02C-8409
GenBank Accession LN489795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112306-0022-132-F02C-8409
GGACTGCTATGCTCCTTGAAATTTTCACAATCTTCAAACCTTCCTTTAACTTAACCTAAT
ATGATCCAAACAAAGAAAGTTAGGCACATGAAAACAAAAACGCTCGTGGAGTAATCAGTA
AAACAAGCCAAAAGATGTCACAAATATTTACCTCTTGTGTGTCTCTGCTGTTGGTAACAG
GTTTGTTCTCATTGTTCTCTAAAGTAATATGCTTCAGTAAACTGTTTGGCACATCCTTCA
CAATATGCCACTTGAGGGGGAAAGAGCCGGTCCACTTATCTTGCTGCCAGTACTCCACAT
TTGTGTTGAAATCAACTGGTCCTGTCATTTCAGCAAGACCAACAAATTGTCCACTTGCAT
TGACCTGAAAGACAAAAACTGGAGTATAAAGAACAATATCATAAAACAAAGTAAAAAGAG
CACAGTTTTTAATTTCAGGATTATATACACACCGAGAAAAACAGAAAGATGGGACAG

Last Updated on 06.12.2018 16:11