Sequences from confirmation amplicon


Confirmation sequences for line 132G01 and hit At4g39240 are shown.
Line ID 132G01
Locus At4g39240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18270509
Primers used for PCR 08vf - 8474, show primer details
   
Sequence Name 47-K117914-0022-132-G01C-08vf
GenBank Accession LN506324 [GenBank]
Sequencing primer gene specific primer, 08vf: 5'-TTATTAACACCCAAACCAGACTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117914-0022-132-G01C-08vf
AGCCTCTCCCATTCCAGACAGAGGGTTACAACCTCCGATTACATACAATTTCCCATCAAA
AAATCCCACGGCGGCGCAGCCACGTGCCTGCTTCATGCTAGGGAGGAGACGACACGTGTG
AGATGGACATTCGACGACAAACCCAGTGGAGACCAATTCATTATGCATCCACCAAGGACG
TAAATTTCAGAATCAATAGCGACAATTGACATTCCCCAGCAAGGAATCGAAGGAAACGAC
GGAATCGGAACCAATCTATGGTTATTTGAATCATTTCCAAAGGGTTTGAAATTGAGAGTG
TACCATCGGGGAGTTTCTGTAGCGTGAGATCTAAGCACAACATAAAGGACACGCTCGGTG
CGGCGAAGTAATGACCTTTCAACGTAGAAATCTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 07-K117913-0022-132-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117913-0022-132-G01C-8409
TCTGTCTGGGAATTGGCTACAGGCTTTAGATCTCAAGACGCATACTTAGTAATCTGGTTG
GTCTATAAAAAAAAAAAGAAAGAGTACTTACCTTTTTTTTTTCAATACAGAAATGTA

Last Updated on 06.12.2018 16:11