Sequences from confirmation amplicon


Confirmation sequences for line 133C11 and hit At1g71870 are shown.
Line ID 133C11
Locus At1g71870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27033059
Primers used for PCR BM03 - 8474, show primer details
   
Sequence Name 11-K015831-022-133-C11C-8409
GenBank Accession LN490893 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K015831-022-133-C11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGAACAATAACT
AGCTCTCTTTTATCTGTGTGTATTTATCATACAAAAGTTTGGCTTCAATAACTTTTTTAA
GATTTGGTTTCATTGATTTTCTCAGGTTGGGAACGAGCTCGGTGCAGGTAGACCTTACAA
GGCCAGACTAGCCGCGAACGTGGCTCTAGCTTGCGCGTTTGTAGTAGGAGCATTGAATGT
GGCTTGGACGGTTATTCTAAAAGAGCGTTGGGCTGGACTATTCACTGGCTATGAGCCACT
CAAGGTCTTGGTTGCTTCGGTTATGCCAATTGTCGGGCTATGCGAGCTAGGGAATTGTCC
ACAGACCACGGGCTGCGGGATTCTTAGAGGGACAGGTCGGCCTGCGGTTGGGGCACATGT
GAATCTCGGGTCGTTTTATTTCGTTGGGACTCCCGTGGCAGTTGGACTAGCGTTTTGGTT
GAAAATTGGGTTTAGTGGGTTGTGGTTTGGGTTGCTTTCANCTCAAGCGGCTTGCGTGGT
TTCGATATTGTATGCGGTTTTGGCGAGGACGGATTGGGAAGGGGAAGCCGTGAAGGCAAT
GAGACTGACGAGTTTTG
   
Sequence Name 12-K015831-022-133-C11C-BM03
GenBank Accession LN491349 [GenBank]
Sequencing primer gene specific primer, BM03: 5'-ATCTCCAAACTCGTCAGTCTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K015831-022-133-C11C-bm03
CGGCTTCCCCTTCCCAATCCGTCCTCGCCAAAACCGCATACAATATCGAAACCACGCAAG
CCGCTTGAGCTGAAAGCAACCCAAACCACAACCCACTAAACCCAATTTTCAACCAAAACG
CTAGTCCAACTGCCACGGGAGTCCCAACGAAATAAAACGACCCGAGATTCACATGTGCCC
CAACCGCAGGCCGACCTGTCCCTCTAAGAATCCCGCAGCCCGTGGTCTGTGGACAATTCC
CTAGCTCGCATAGCCCGACAATTGGCATAACCGAAGCAACCAAGACCTTGAGTGGCTCAT
AGCCAGTGAATAGTCCAGCCCAACGCTCTTTTAGAATAACCGTCCAAGCCACATTCAATG
CTCCTACTACAAACGCGCAAGCTAGAGCCACGTTCGCGGCTAGTCTGGCCTTGTAAGGTC
TACCTGCACCGAGCTCGTTCCCAACCTGAGAAAATCAATGAAACCAAATCTTAAAAAAGT
TATTGAAGCCAAACTTTTGTATGATAAATACACACAGATAAAAGAGAGCTAGTTATTGTT
CTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATNGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TT

Last Updated on 06.12.2018 16:11