Sequences from confirmation amplicon


Confirmation sequences for line 133F03 and hit At4g20170 are shown.
Line ID 133F03
Locus At4g20170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10898515
Primers used for PCR CD56 - 8474, show primer details
   
Sequence Name 07-K104268-0022-133-F03C-CD56
GenBank Accession LN489252 [GenBank]
Sequencing primer gene specific primer, CD56: 5'-TTACATGTTCCAGTGAAAGAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K104268-0022-133-F03C-CD56
GAATTCTCGTTACTATTGAAAGATGCCTATGAGTAGTCGGATTTGTTATTCCGGTGATGG
TCCGGCGAGAACTTACAGGTAACATTCATAACTTTGTTGATTTTAATGATTGAACGTTAA
CCAAATCCCATGTGTTGTTTATTGAGTGACTTAGAATATTAGTCACAAGTTTTTTGAAGG
TGACATATAATATCAATAAGTATCTGATTCGGTCAACACTTGGCCTGTAAAAGTCTTTGT
GCATTCTGTATTAGTCTCCTATGTCGGTCACGATCTTTACTTTTATTGAACATCACATGA
TTTTACTGAGAAATGTTTTCACATGATTTTAGTGAGAAATGTTTTCACATGATTTTAGTG
AGAAATGTTTTAAAGATTCTATCTTTTGTTTGTATCTTTTTTGTTTAATCAGAATAACAA
TTGAATTGTGATGTAGATTAGTTTAGTATTGAACATCTCTAATGGTTCATCTTAGGAGGG
AACATATCGGTTATTGATTTGGTTTAGTTCAGGGACTGATCTAAATGGTATTTGGTTTTG
TGTTTCAGGAAATGGGGAATTGAGAAACTGGCATATAGAGACGTCAAGAAGGTTCCAAGA
CGGGATCGAAAATACCAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K104269-0022-133-F03C-8409
GenBank Accession LN496537 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104269-0022-133-F03C-8409
TCATTGGTATTTTCGATCCCGTCTTGGAACCTTCTTGACGTCTCTATATGCCAGTTTCTC
AATTCCCCATTTCCTGAAACACAAAACCAAATACCATTTAGATCAGTCCCTGAACTAAAC
CAAATCAATAACCGATATGTTCCCTCCTAAGATGAACCATTAGAGATGTTCAATACTAAA
CTAATCTACATCACAATTCAATTGTTATTCTGATTAAACAAAAAAGATACAAACAAAAGA
TAGAATCTTTAAAACATTTCTCACTAAAATCATGTGAAAACATTTCTCACTAAAATCATG
TGAAAACATTTCTCAGTAAAATCATGTGATGTTCAATAAAAGTAAAGATCGTGACCGACA
TAGGAGACTAATACAGAATGCACAAAGACTTTTACAGGCCAAGTGTTGACCGAATCAGAT
ACTTATTGATATTATATGTCACCTTCAAAAAACTTGTGACTAATATTCTAAGTCACTCAA
TAAACAACACATGGGATTTGGTTAACGTTCAATCATTAAAATCAACAAAGTTATGAATGT
TACCTGTAAGTTCTCGCCGGACCATCACCGGAATAACAAATCCGACTACTCATAGGCATC
TGTTCAATAGTAAACTGAGAATATTCCTCCAAAGATTCCATCACAGACGAAATCGTCTCT
NTCACTGGAACATGT

Last Updated on 06.12.2018 16:11