Sequences from confirmation amplicon


Confirmation sequences for line 134A01 and hit At5g20850 are shown.
Line ID 134A01
Locus At5g20850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7071867
Primers used for PCR BR01 - 8474, show primer details
   
Sequence Name 01-K031137-022-134-A01C-8409
GenBank Accession LN486452 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K031137-022-134-A01C-8409
TTCCAGAGCGGAACATCACCATATAACTTTGTGATGGAACCCGTTTCAATACCTCCTGTT
GAGCATACGGCAAAGTAAGATCACTATAACAAATTGCCGTTATAAAATGCACCATAAATC
AGCACCAACCTTCTAGAATTTTCTTTAGCTCCCGTGATCCAGAGTTAATCTGAATAATTT
CCTGTCTCTGAACATGGCAGCTGGCTCGCACTAGTGAATCCCAGAGGAACTAGCTTTGAA
GCTATTACAGATGAAAACACAAATTGCAACACACATTCACTCTAATAGAATCAGAGAGGT
GTTATGCATTTAACAGAGAAGGAAAGCAAAAAGGTTCTACATGTGTAATACCTGCAACGA
CTATCTTGTCAACCTTGTCATCACTAATGTACTTTAATCTGCAAGAGATCCTTCTCTCGG
AGTATAAGCAACACCTTCAACAGTACAGAGACCAGCATCCCTAAGCTTCTTTACATCAAC
AGAAGCAATACCTGCATAGAGTGCTATGTTTACCATGTTGGACCATTTATTTTTACTCTG
ACTCTATATTATATACCTAATATGAGAACAATT
   
Sequence Name 36-K031137-022-134-A01C-BR01
GenBank Accession LN502106 [GenBank]
Sequencing primer gene specific primer, BR01: 5'-GCAGGTATTGCTTCTGTTGATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K031137-022-134-A01C-BR1L
GTTGAGGTGTTGCTTATACTCCGAGGAAGGATCTCTTGCAGATTAAAGGAATTAGTGATG
CCAAGGTTGACAAGATTGTAGAAGCAGGTATTACACATGTAGAAACTTTTTGCTTTCCTT
CTCTGTTAAATACATAACACCTCTTTGATACTCTTAGAGTTAATGTGTGTTCTTATTTGT
GTTTTCTTCTGTGATAGCTTCAAAGCTAGTTCCTCTGGGGTTCACTAGTGCGAGCCAGCT
CCATGCTCAGAGACAGGAAATTATTCAGATTACCTCTGGATCACGGGAGCTCGATAAAGT
TCTAGAAGGTTGGTGCTGGTTTCTGGTGCATCTTATAACGGCATTTTGTTATTGTGATCT
TACTTTGCCGTATGCTCAACAGGAGGTATTGAAACTGGTTCCATCACAGAGTTATATGGT
GAGTTCCGCTCTGGAAAGACTCAGCTGTGCCATACACTGTGTGTGACTTGTCAAGTAAGA
ACCCTGTTCCTATACCACTCAGTTATTGTTGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTNTATNTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11