Sequences from confirmation amplicon


Confirmation sequences for line 134B08 and hit At3g46420 are shown.
Line ID 134B08
Locus At3g46420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17082899
Primers used for PCR NR72 - 8474, show primer details
   
Sequence Name 01-K102660-0022-134-B08C-8409
GenBank Accession LN486526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K102660-0022-134-B08C-8409
CGGAATGAGCCTTTACAATTGAATATATCCTGTATTGCTGATCCATATATGGGAATTATG
ATGGTCTTAACATTTATCCTAAATTTGACTTGTACATAGGCCCTAATTTTTGGGTAACAA
TAGACTTAGGAAAGTATGTAAATGGTACATGGGAGGAGATCATCTACATACCAAAATCTA
ATATGTTGGACGTGTGTCTTGTCAAGACAGGGCCGTCCACACCATTGATATCATCCTTGG
TGTTAAGGCCTCTTGCAAATGCTACTTACATCACTCAATCTGGTTGGTTGAAGACTTATG
TTCGGGTTTATCTAAGCGACTCAAATGACGTCATACGGTAAGTCATCTTAAAGAAACGCG
GACTC
   
Sequence Name 20-K102661-0022-134-B08C-NR72
GenBank Accession LN495165 [GenBank]
Sequencing primer gene specific primer, NR72: 5'-CCGCGTTTCTTTAAGATGACTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102661-0022-134-B08C-NR72
TGATACCCGAACTAAGTCTTCACCAACCAGATTGAGTGATGTAAGTAGCATTTGCAAGAG
GCCTTAACACCAAGGATGATATCAATGGTGTGGACGGCCCTGTCTTGACAAGACACACGT
CCAACATATTAGATTTTGGTATGTAGATGATCTCCTCCCATGTACCATTTACATACTTTC
CTAAGTCTATTGTTACCCAAAAATTAGGGCCTATGTACAAGTCAAATTTAGGATAAATGT
TAAGACCATCATAATTCCCATATATGGATCAGCAATACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACNTTCACTC
CCAAATGG
Primers used for PCR NR73 - 3144, show primer details
   
Sequence Name 02-K102660-0022-134-B08C-3144
GenBank Accession LN486981 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K102660-0022-134-B08C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAAACC
TACATGATATCCGGGAATGTAGCTCTGATTATCCATATTGCTGATCCATCATAATTCCTT
GTGTCACTGTAAGATTGTAACAGTTGCGTATTCCATCAGGAAAATATCTCAACGTCGTTT
GCGACTTTCGGTACTTCGGCTCTAGGCTTGCCTGAATTCTACCAAGTTTTCCGGTTTCAA
TGAAATTTGAATCCGTTGTGAACTGTAGTCCAGTAAACGGCTCAATATAAGGAGAGACCT
CATTAGGAGATAATCCGCAGTCTACACTGATAAATCCTGTTAAGATATTAAAATCGTATT
ACCAAAACTAGATAAAAATTCCTCATCAAACTATCCCATTATATAAATAAAGGATTAGTT
TGTGAACTAAGACCTTTTTTAACTTATATTAGTAATCTAGCATGCACACAAAAAGG
   
Sequence Name 21-K102661-0022-134-B08C-NR73
GenBank Accession LN495615 [GenBank]
Sequencing primer gene specific primer, NR73: 5'-CCTTTTTGTGTGCATGCTAGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K102661-0022-134-B08C-NR73
ACTAATCCTTTATTTATATAATGGGATAGTTTGATGAGGAATTTTTATCTAGTTTTGGTA
ATACGATTTTAATATCTTAACAGGATTTATCAGTGTAGACTGCGGATTATCTCCTAATGA
GGTCTCTCCTTATATTGAGCCGTTTACTGGACTACAGTTCACAACGGATTCAAATTTCAT
TGAAACCGGAAAACTTGGTAGAATTCAGGCAAGCCTAGAGCCGAAGTACCGAAAGTCGCA
AACGACGTTGAGATATTTTCCTGATGGAATACGCAACTGTTACAATCTTACAGTGACACA
AGGAATTATGATGGATCAGCAATATGGATAATCAGAGCTACATTCCCGGATATCATGTAG
GTTTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG
TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTC

Last Updated on 06.12.2018 16:11