Sequences from confirmation amplicon


Confirmation sequences for line 134C06 and hit At5g65810 are shown.
Line ID 134C06
Locus At5g65810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26338178
Primers used for PCR 19fb - 8474, show primer details
   
Sequence Name 47-K111470-0022-134-C06C-19fb
GenBank Accession LN506230 [GenBank]
Sequencing primer gene specific primer, 19fb: 5'-TGACAAGCTGGTTTGTATGAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111470-0022-134-C06C-19fb
GCTGCTGCTTCGTTTCCTCTAGTTCGTCTGTGAGAAGAAACGGATCCACCACGACGAGCT
ACGCATTTTAGCCTGCAAGACATTGGAATTGCAACAAGTTAGCAAACAGGACAAGTACGA
ACCTAACCCACATCTCTGAATTGATGAATGTAATGTGTGGTTTCATTCATGACGAGAACA
TGGTTTTAGCGAACTTACAGGCCGTCCAAATTTCGACAATTCCCCACCTTTAGCCTTTTG
TTGACCAGGGTTACCTATATAGTAGTAACGGGAAATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 31-K111469-0022-134-C06C-8409
GenBank Accession LN500218 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K111469-0022-134-C06C-8409
TATTTCCCGTTACTACTATATAGGTAACCCTGGTCAACAAAAGGCTAAAGGTGGGGAATT
GTCGAAATTTGGACGGCCTGTAAGTTCGCTAAAACCATGTTCTCGTCATGAATGAAACCA
CACATTACATTCATCAATTCAGAGATGTGGGTTAGGTTCGTACTTGTCCTGTTTGCTAAC
TTGTTGCAATTCCAATGTCTTGCAGGCTAAAATGCGTAGCTCGTCGTGGTGGATCCGTTT
CTTCTCACAGACGAACTTAGAGGAAAACGAAGCAGCAAGCAAGAAATTCGAACAAGCAGC
TTCCAAGAGTTCATACAAACCAGCTTGTC

Last Updated on 06.12.2018 16:11