Sequences from confirmation amplicon


Confirmation sequences for line 134C07 and hit At5g61200 are shown.
Line ID 134C07
Locus At5g61200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24621288
Primers used for PCR MF20 - 8474, show primer details
   
Sequence Name 14-K031809-022-134-C07C-MF20
GenBank Accession LN492392 [GenBank]
Sequencing primer gene specific primer, MF20: 5'-TCGTTGTCAGAGTCTCGTTTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031809-022-134-C07C-MF20
GATTGTTGATTGCTACCAGGAGATTGGTAATGGAGAGATTCTCATGAAACTGAAATCTAG
GCATATATTTTATATGAGCTAAAAAACACTTTCTTTGTCTTTCAGATTATTTGCGGGATC
AAGTAAACTTCAGAAGCCAGGAAATGAACGACCTGAGTGAACATGTACTCGATCTTGAAG
TCAGAGTAACTAAATCAGGGAAATTGGAAGAAGAAGTTAACTATTTGAGGGAGGAGTTGT
GTTCGTCGAAATCTGAGCAGCTATTGTTGTTGCAAGAACTGGAGAGCACAGAAACTGAGC
TACAGTTTTCTTTGTTTTCTGTTGAGAAGCTGGAGGAGTCGGTCTCATCCTTAACATTGG
AGTCTCAGTGTGAGATAGAAAGCATAAAACTTGATATAGTAGCGTTGGAGCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 13-K031809-022-134-C07C-8409
GenBank Accession LN491920 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K031809-022-134-C07C-8409
ATATCAGTTTATGCTTTCTATCTCACACTGAGACTCCAATGTTAAGGATGAGACCGACTC
CTCCAGCTTCTCAACAGAAAACAAAGAAAACTGTAGCTCAGTTTCTGTGCTCTCCAGTTC
TTGCAACAACAATAGCTGCTCAGATTTCGACGAACACAACTCCTCCCTCAAATAGTTAAC
TTCTTCTTCCAATTTCCCTGATTTAGTTACTCTGACTTCAAGATCGAGTACATGTTCACT
CAGGTCGTTCATTTCCTGGCTTCTGAAGTTTACTTGATCCCGCAAATAATCTGAAAGACA
AAGAAAGTGTTTTTTAGCTCATATAAAATATATGCCTAGATTTCAGTTTCATGAGAATCT
CTCCATTACCAATCTCCTGGTAGCAATTCAACAATTCTTTCTCCAACATCTGAATTCTTC
TCTTATCTTCTAAACGAGACTTGNACAAC

Last Updated on 06.12.2018 16:11