Sequences from confirmation amplicon


Confirmation sequences for line 134D01 and hit At1g22220 are shown.
Line ID 134D01
Locus At1g22220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7846996
Primers used for PCR BG44 - 8474, show primer details
   
Sequence Name 69-K101747-0022-134-D01C-8409
GenBank Accession LN512165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101747-0022-134-D01C-8409
GCCTTTACATTGAATTATCCTGAAGAGAATATATAGAGGAAGACTCGGGTACAATTATAA
CGCGAATATATATTCTATGTCCAGCATATTGACCTTTCTCAATCCGGTCGAACCGGGAGA
GAATCTTGAAGGGGAGACTCGGAGAGAGGGTGGTTATTGTGATTGGTTTAGCCGGTTTGG
ATAAAAGAGAAACTAGACCGTTGAATAAAGATCGGAAAATGCTTGTGACGACGGGTGAAT
CGGATACTGAGTGGTCGAGTCGGAGGGTGAGTGACTCAGACTGAGGGACAAGCGAGTTAA
ATCTTTTTGAAAGAGAGCTGCATCGAAGTAAGGTTTTGACGTCACCGACTTTGTTCAAGA
TGTCGACGATGATCGGGTCAGGAAGTCCATCAAAAACGTCCATCTTACAAAACCTTTTGC
AAACAAAAAAAAGAGAGAGTCTCTTTGTTTCTCTGTGTTTTCTTCTGTTTCACTTTCTTG
CTATGATTGTATTATGCTTGGAGTTTGGAGAGTGTAACAAAGGAGTTTAAATAGAGGCGT
TTTTGGAAAAGGAAGAATAAGCCNAAAACCAA
   
Sequence Name 69-K101753-0022-134-D01C-BG44
GenBank Accession LN512166 [GenBank]
Sequencing primer gene specific primer, BG44: 5'-ATTGGTTTTTGGCTTATTCTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101753-0022-134-D01C-BG44
TTACTCCTTTGTTCACTCTCCAACTCCAAGCATAATACAATCATAGCAAGAAAGTGAAAC
AGAAGAAAACACAGAGAAACAAAGAGACTCTCTCTTTTTTTTGTTTGCAAAAGGTTTTGT
AAGATGGACGTTTTTGATGGACTTCCTGACCCGATCATCGTCGACATCTTGAACAAAGTC
GGTGACGTCAAAACCTTACTTCGATGCAGCTCTCTTTCAAAAAGATTTAACTCGCTTGTC
CCTCAGTCTGAGTCACTCACCCTCCGACTCGACCACTCAGTATCCGATTCACCCGTCGTC
ACAAGCATTTTCCGATCTTTATTCAACGGTCTAGTTTCTCTTTTATCCAAACCGGCTAAA
CCAATCACAATAACCACCCTCTCTCCGAGTCTCCCCTTCAAGATTCTCTCCCGGTTCGAC
CGGATTGAGAAAGGTCAATATGCTGGACATAGAANATATATTCGCGTTATAATTGTACCC
GAGTCTTCCTCTATATATTCTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11