Sequences from confirmation amplicon


Confirmation sequences for line 134D01 and hit T4M8 are shown.
Line ID 134D01
Locus T4M8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1076479
Primers used for PCR KN43 - 3144, show primer details
   
Sequence Name 06-K103223-0022-134-D01C-3144
GenBank Accession LN488768 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103223-0022-134-D01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAA
AGCAATTTTGGAAGAATTAAAACCACTTTGAAAACCACAACTATTCAAAACCATAATTTC
CTTGGAAAAATAAAGATTTTCATAAAAACCTCAAAACCATGGTTTTTAGTTTCTTTTAGA
AACTTGGAGAAATCACTAGAAAACTAGTTTCCCAAAATTTTAAATAAAAAAATCATTTTT
TAGAAATCTTTGTAAAAACTACCTTGGTCCATTGGTTAACCAAGTATTAAATAATTTTTC
TAGACCTAGAGGACCGCTNAAAACTAGTAAAACATTGAATGCCAATTGTG
   
Sequence Name 54-K103230-0022-134-D01C-KN43
GenBank Accession LN508444 [GenBank]
Sequencing primer gene specific primer, KN43: 5'-CACAATTGGCATTCAATGTTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103230-0022-134-D01C-KN43
AATTATTTATACTTGGTTACCAATGGACCAAGGTAGTTTTTACAAAGATTTCTAAAAAAT
GATTTTTTTATTTAAAATTTTGGGAAACTAGTTTTCTAGTGATTTCTCCAAGTTTCTAAA
AGAAACTAAAAACCATGGTTTTGAGGTTTTTATGAAAATCTTTATTTTTCCAAGGAAATT
ATGGTTTTGAATAGTTGTGGTTTTCAAAGTGGTTTTAATTCTTCCAAAATTGCTTTTCAA
ACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCT
CTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTG
TGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGT
TGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGG

Last Updated on 06.12.2018 16:11