Sequences from confirmation amplicon


Confirmation sequences for line 134E12 and hit At5g18290 are shown.
Line ID 134E12
Locus At5g18290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6056309
Primers used for PCR ML21 - 8474, show primer details
   
Sequence Name 03-K100818-0022-134-E12C-8409
GenBank Accession LN487400 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K100818-0022-134-E12C-8409
CATTTACACGCCGTAGGGTTTCTGGTATCACTTGGGCTCCTCTTGTGATCTCAACGTTGG
TTGTTTTTGTCTCCATCTCGATTTTTACGGTTATTGGAAACGTTCTTGGTGGTGCTAGCT
TCAATCCTTGTGGAAACGCAGCGTTTTATACCGCCGGTGTTTCCAGTGATTCTCTCTTCT
CCTTGGCCATTAGATCTCCTGCTCAGGTGTGTGTTTATGATCTAAGCTAGCTTTTGAAAC
CAAACCAACTTCTTCTTCTTGTTCTTTATTTTGTTCTGAACAGAAAGATTTTTGTTCATA
TACTTGTGACATCTTTTCATATGAGTATTTGCTTTTGTGTCTTGTCTCTTCGAATAAGAA
TCTATTAAAGTTTTATCCTTACACATTGTTTATGAATTTCTTTACATTTATTTTGTTGTT
AGAGCAATGTATTTTCTTTGTTTGTTGGATCTATGTGAAGTTTATTGTGTGTGGTCCATG
TGTGTTTTTGAATAACGTACAAACTTTTTTAATTGGAGATTTTGGCCCAAGTACCCACTG
GCATATTTGTTT
   
Sequence Name 03-K100830-0022-134-E12C-ML21
GenBank Accession LN487402 [GenBank]
Sequencing primer gene specific primer, ML21: 5'-GAAACAAATATGCCAGTGGGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K100830-0022-134-E12C-ML21
GTTATTCAAAAACACACATGGACCACACACAATAAACTTCACATAGATCCAACAAACAAA
GAAAATACATTGCTCTAACAACAAAATAAATGTAAAGAAATTCATAAACAATGTGTAAGG
ATAAAACTTTAATAGATTCTTATTCGAAGAGACAAGACACAAAAGCAAATACTCATATGA
AAAGATGTCACAAGTATATGAACAAAAATCTTTCTGTTCAGAACAAAATAAAGAACAAGA
AGAAGAAGTTGGTTTGGTTTCAAAAGCTAGCTTAGATCATAAACACACACCTGAGCAGGA
GATCTAATGGCCAAGGAGAAGAGAGAATCACTGGAAACACCGGCGGTATAAAACGCTGCG
TTTCCACAAGGATTGAAGCTAGCACCACCAAGAACGTTTCCNATAACCGTNAAAATCGAG
ATGGAAGACAAAACACCAACGTTGAGATCACAAGAGGAGCCCAAGTGATACCATGAAACC
CTACNGCGTTGTAAATGGCTTCATGTC

Last Updated on 06.12.2018 16:11