Sequences from confirmation amplicon


Confirmation sequences for line 134G01 and hit At5g41620 are shown.
Line ID 134G01
Locus At5g41620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16648163
Primers used for PCR 22ra - 8474, show primer details
   
Sequence Name 27-K116053-0022-134-G01C-22ra
GenBank Accession LN498540 [GenBank]
Sequencing primer gene specific primer, 22ra: 5'-CCACTCTGGATAGATTAGCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116053-0022-134-G01C-22ra
CTTAGTTTTCAAGTATTATCCTTTACTCCATGAGCTATCGCAATCTCCGACGGGCCTAGA
AGATCAGGTGCGACGGTTCTTGAAATCCATTGTCGAACTGGACTTGCTTGTCTTCGAGAA
GATGGGAAATTGCAAGACGCTTCATCGATTTCACGAGTTTCGGACAATAACCTTCTGTGT
GTTCGTATCATTTCCCCCATTACATCGTTCTTGTTCGTAGTAGCACACTCATTTTCTGGT
TTCTTATTGTTGTTGCTGTTCTCAGGCTTTACATCTTCTTCTTCTTCTTCGTCTTCAATA
GCTCGGGTGGTTTTCTTCTTTCCGTTACTCGATGACGCCCACGCCATTTGATCCTCAAAG
TTCACTTGGAAACTCGACGGGCTTTTCGGCTTTTCATCAATCGAACCGTCTTTGTTATGT
TGGTTTGGTTTCTTAGTTTCGTCTCCATAAGACTCTGCAGGTTTCTTGAGCTCAAAGCAA
TTAGAATCGCTTCCTCCATAGTCTTCTTCACAGTCAACATCCCGCGGTGGTGCACTCAAG
GTGTTAAACGGAACAGATTCAAGTGAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGA
   
Sequence Name 10-K116052-0022-134-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116052-0022-134-G01C-8409
ATTACTTGATCTGTTCCGTTTACACCTTGAGTGCGCGACCGCGGGATGT

Last Updated on 06.12.2018 16:11