Sequences from confirmation amplicon


Confirmation sequences for line 136B02 and hit At2g20800 are shown.
Line ID 136B02
Locus At2g20800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8954531
Primers used for PCR ML19 - 8474, show primer details
   
Sequence Name 60-K100940-0022-136-B02C-8409
GenBank Accession LN510202 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K100940-0022-136-B02C-8409
GCCATTTACAATTGAATATATCCTGAAGCAGGCGACCACATTCTCAACATGTAAGTTCCA
CCCATCAAGCTTCATTAACTTTGGCTAAATCTACTGCTTTTCCAATGTTGGTTCAATTCT
ACTTCAGGTTTGACAAGAGAATCACTGCTTTTGCTGAGGAAAAGTTCCAGCGAGATGGTA
TTGATCTGAAGACAGGTTCAATGGTTGTGGGAGTGACTGCTGATGAGATATCCACAAAGG
AAAGAGAAACTGGCAAGATTGTCTCCGAACCTTATGGTATGGTTGTGTGGTCAACAGGAA
TTGGTTCACGTCCTGTCATCAAAGACTTTATGCAGCAGATTGGTCAGGTTAGATTACACT
TGATCACATGAAGAAATCATTTGACTAACCAAATCATTTGCTAGTGTCCCACCTAACTAG
ATNGAGTATGGTTACAGGGACAGAGACGTGTCTTGGCTACCGACGAATGGCTTAGAGAAA
GGGGATGTG
   
Sequence Name 60-K100947-0022-136-B02C-ML19
GenBank Accession LN510203 [GenBank]
Sequencing primer gene specific primer, ML19: 5'-TCACATCCCTCTACTCTAAGCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K100947-0022-136-B02C-ML19
ACCATACTCAATCTAGTTAGGTGGGACACTAGCAAATGATTTGGTTAGTCAAATGATTTC
TTCATGTGATCAAGTGTAATCTAACCTGACCAATCTGCTGCATAAAGTCTTTGATGACAG
GACGTGAACCAATTCCTGTTGACCACACAACCATACCATAAGGTTCGGAGACAATCTTGC
CAGTTTCTCTTTCCTTTGTGGATATCTCATCAGCAGTCACTCCCACAACCATTGAACCTG
TCTTCAGATCAATACCATCTCGCTGGAACTTTTCCTCAGCAAAAGCAGTGATTCTCTTGT
CAAACCTGAAGTAGAATTGAACCAACATTGGAAAAGCAGTAGATTTAGCCAAAGTTAATG
AAGCTTGATGGGTGGAACTTACATGTTGAGAATGTGGTCGCCTGCTTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG

Last Updated on 06.12.2018 16:11