Sequences from confirmation amplicon


Confirmation sequences for line 136C11 and hit At1g18690 are shown.
Line ID 136C11
Locus At1g18690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6436034
Primers used for PCR BK45 - 8474, show primer details
   
Sequence Name 06-K104526-0022-136-C11C-BK45
GenBank Accession LN488798 [GenBank]
Sequencing primer gene specific primer, BK45: 5'-GAATCAGAACCCTGAGTTTCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104526-0022-136-C11C-BK45
CTTCTCACAGGGTCTTCCCCCGGACCCTGTGATAAACCAATTGGAAACTATTATCTATTG
AAAGCTGTGAAGAACAAGATTGATTACTGTAGACTCCACGGGATAGAGATTGTGTATAAC
ATGGCGAATTTGGATGAGGAACTCTCAGGGTATTGGACGAAATTACCTATGATTAGGACA
TTAATGCTGTCTCATCCAGAAGTAGAATGGATTTGGTGGATGGATAGTGATGCTTTGTTC
ACTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 22-K104527-0022-136-C11C-8409
GenBank Accession LN496099 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104527-0022-136-C11C-8409
GCCATTTACATTGAATATATCAGTGAACAAAGCATCACTATCCATCCACCAAATCCATTC
TACTTCTGGATGAGACAGCATTAATGTCCTAATCATAGGTAATTTCGTCCAATACCCTGA
GAGTTCCTCATCCAAATTCGCCATGTTATACACAATCTCTATCCCGTGGAGTCTACAGTA
ATCAATCTTGTTCTTCACAGCTTTCAATAGATAATAGTTTCCAATTGGTTTATCACAGGG
TCCGGGGGAAGACCCTGTGAGAAGCAATATTCGAGCTTTGCCATTGACAATATTAGGAAA
CTCAGGGTTCTGATT

Last Updated on 06.12.2018 16:11