Sequences from confirmation amplicon


Confirmation sequences for line 136F09 and hit At5g43330 are shown.
Line ID 136F09
Locus At5g43330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17391506
Primers used for PCR GG31 - 8474, show primer details
   
Sequence Name 85-K100901-0022-136-F09C-8409
GenBank Accession LN514511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K100901-0022-136-F09C-8409
CATTTACAATTGAATATATCCTGATAGGATCAATGCATTGGTGTTTGCAGGGTTTGCAAC
AACCAGAACCTACAAAACGAAGAGATAGCAAATATGAAATATCTCATACAACAAAATAAG
AGATTAACTTGCTTAATTTCTGCATAATGATCATAAGAACGATTCGAAAAGACTTATCAC
CTTGCAGTTTGGTGCAGCATGCTTCTCCAAGGCAGAAGCTTGAGACTTGTAAATGGAAAC
GTTCTTGGACATAACATCCTTCCTCTCCATTCCTTCCTTCCTCGGGAAACCACCAACCAT
GACAGCAACATTAACACCGGTACACGCTTCAACAGCATCAGTTGTAGCCACCACACCTTC
CACAGAAAAAACACCAACATCACATTTCAGCCTCAAGCAACATACCATATTCCAATACGA
GCACAGTAAAAATATATACTAGAATAAAAACTTACCCTTTAGAAGAGGGAAAGCAGCATC
GACCAATTCCATCTTCACACCGTTCAAAGCTTCTGCTGCAAAAGGGATGTCAAGCATGTG
GAGGATCACAGGCTGATCAGCACCAAGCATTATCCCCCTTGCAATCATAGGAACAAGAGC
ATATCCGATTTGTCCTGCACAATA
   
Sequence Name 85-K100907-0022-136-F09C-GG31
GenBank Accession LN514512 [GenBank]
Sequencing primer gene specific primer, GG31: 5'-AGTGTGTTTCTGTTATTGTGCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K100907-0022-136-F09C-GG31
ATTGCAGGGGGATAATGCTTGGTGCTGATCAGCCTGTGATCCTCCACATGCTTGACATCC
CTTTTGCAGCAGAAGCTTTGAACGGTGTGAAGATGGAATTGGTCGATGCTGCTTTCCCTC
TTCTAAAGGGTAAGTTTTTATTCTAGTATATATTTTTACTGTGCTCGTATTGGAATATGG
TATGTTGCTTGAGGCTGAAATGTGATGTTGGTGTTTTTTCTGTGGAAGGTGTGGTGGCTA
CAACTGATGCTGTTGAAGCGTGTACCGGTGTTAATGTTGCTGTCATGGTTGGTGGTTTCC
CGAGGAAGGAAGGAATGGAGAGGAAGGATGTTATGTCCAAGAACGTTTCCNTTTACAAGT
CTCAAGCTTCTGCCTTGGAGAAGCATGCTGCACCAAACTGCAAGGTGATAAGTCTTTTCG
AATCGTTCTTATGATCATTATGCAGAAATTAAGCAAGTTAATCTCTTATTTTGTTGTATG
AGATATTTCATATTTGCTATCTCTTCGTTTTGTAGGTTCTGGTTGTTGCAAACCCTGCAA
ACACCAATGCATTGATCCTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCNGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAGAAATGT

Last Updated on 06.12.2018 16:11