Sequences from confirmation amplicon


Confirmation sequences for line 136H02 and hit At5g47430 are shown.
Line ID 136H02
Locus At5g47430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19236666
Primers used for PCR 04jr - 8474, show primer details
   
Sequence Name 20-K113214-0022-136-H02C-8409
GenBank Accession LN495287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K113214-0022-136-H02C-8409
ATTTACATTGTAATAGAAGGGTAGATGGCTAAAAAGCCTAAAATGCAAAATAGTAGGCGT
AGGAACCGAAAGGTGACGACGGTAACTGGGCTATTGAGCCCATTTCCTTTTTATTTGTGG
GCCGAATTCAATACATATTTTATTATAATAATTTGGGCTAACAATTGTTTTACAGAGACA
AGCACCTCCACCACCAATCCACGATTACGATCGCCGGAGACGACCGGAGAAGAGACTATC
CCCAGAGCATCCGCCTACGCGTAAAAACATTTCCCCGTCTCGCGACTCCAAGAGAAAATC
CGAGCGTTATCCTGATGAACGAGATCGGCAACGCGACCGTGAGAGGTCACGTCACCAAGA
CGTAGATCGTGAACACGACCGTACTCGCGACCGTCGCGATGAAGACAGAAGCCGAGATCA
TCGCCACCACCGTGGTGAAACCGAACGCAGCCAACATCACCACCGCAAGAGATCGGAACC
GCCTTCATCGGAGCCACCAGTCCCTGCTACTAAAGCAGAGATCGAGAATAATCTTAAGTC
TAGCGTATTCGCTCGGATAAGTTTTCCGGAGGAAGAAACATCTTCTGGTAAACGAAGAAA
GG
   
Sequence Name 20-K113215-0022-136-H02C-04jr
GenBank Accession LN495288 [GenBank]
Sequencing primer gene specific primer, 04jr: 5'-CCTTTCTTCGTTTACCAGAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K113215-0022-136-H02C-04jr
GATACGCTAGACTTAGATTATTCTCGATCTCTGCTTTATAGCAGGGACTGGTGGCTCCGA
TGAAGGCGGTTCCGATCTCTTGCGGTGGTGATGTTGGCTGCGTTCGGTTTCACCACGGTG
GTGGCGATGATCTCGGCTTCTGTCTTCATCGCGACGGTCGCGAGTACGGTCGTGTTCACG
ATCTACGTCTTGGTGACGTGACCTCTCACGGTCGCGTTGCCGATCTCGTTCATCAGGATA
ACGCTCGGATTTTCTCTTGGAGTCGCGAGACGGGGAAATGTTTTTACGCGTAGGCGGATG
CTCTGGGGATAGTCTCTTCTCCGGTCGTCTCCGGCGATCGTAATCGTGGATTGGTGGTGG
AGGTGCTTGTCTCTGTAAAACAATTGTTAGCCCAAATTATTATAATAAAATATGTATTGA
ATTCGGCCCACAAATAAAAAGGAAATGGGCTCAATAGCCCAGTTACCGTCGTCACCTTTC
GGTTCCTACGCCTACTATTTTGCATTTTAGGCTTTTTAGCCATCTACCCTTCTATTTACA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA

Last Updated on 06.12.2018 16:11