Sequences from confirmation amplicon


Confirmation sequences for line 137C12 and hit At1g53910 are shown.
Line ID 137C12
Locus At1g53910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20136315
Primers used for PCR IW19 - 8474, show primer details
   
Sequence Name 33-K016212-022-137-C12C-8409
GenBank Accession LN500785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K016212-022-137-C12C-8409
ATTTGGTTGGAGCGATCAAGCTCCGATAACTCCCGACATCTCTTCTGCGGTTATCAACAA
CAACAACTCAGCTCTGTTCTTTGAGGAAGCCAATCCAGCTAAGAAGCTCAAGTCTATGGA
TTTCGAGACACCTTACAACAACACTGAATGGGACGCTTCACTGGATTTCCTCAACGAAGA
TGCTGTAACGACTCAGGACAATGGTGCAAACCCTATGGACCTATGGAGTATTGATGAAAT
TCATTCCATGATTGGAGGAGTCTTCTGAAGAGATCCAGTTTCATGTAAATAAGGCTGCAT
GTTTGTGAGTTTCCCGCATCGTTCGTTTATCAACCTCCAAAACTTTCTAATGTCTGTTAC
TTGCATCTTCTTCTGCTGTCTCTGTCTGTCTCTCTCAGGAGTTCCTGTTTGCATTGCGAG
AAGCCATGAGCCTCTATCTTGAGGGTAGTTGTGATGAAGTTAAGTAGAGGCTTATTTTTA
GGGGTTGTGGTAGTTTTTGTTTTAGTGAATCTTTTGAATTCGTTTGTGTTTTGTTTTTGT
TACTTT
   
Sequence Name 34-K016212-022-137-C12C-IW19
GenBank Accession LN501207 [GenBank]
Sequencing primer gene specific primer, IW19: 5'-TTTGGGGCATAAAGTAACAAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K016212-022-137-C12C-iw19
CAAACGAATTCAAAAGATTCACTAAAACAAAAACTACCACAACCCCTAAAAATAAGCCTC
TACTTAACTTCATCACAACTACCCTCAAGATAGAGGCTCATGGCTTCTCGCAATGCAAAC
AGGAACTCCTGAGAGAGACAGACAGAGACAGCAGAAGAAGATGCAAGTAACAGACATTAG
AAAGTTTTGGAGGTTGATAAACGAACGATGCGGGAAACTCACAAACATGCAGCCTTATTT
ACATGAAACTGGATCTCTTCAGAAGACTCCTCCAATCATGGAATGAATTTCATCAATACT
CCATAGGTCCATAGGGTTTGCACCATTGTCCTGAGTCGTTACAGCATCTTCGTTGAGGAA
ATCCAGTGAAGCGTCCCATTCAGTGTTGTTGTAAGGTGTCTCGAAATCCATAGACTTGAG
CTTCTTAGCTGGATTGGCTTCCTCAAAGAACAGAGCTGAGTTGTTGTTGTTGATAACCGC
AGAAGAGATGTCGGGAGTTATCGGAGCTTGATCGCTCCAACCAAACTAAGAAAAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11