Sequences from confirmation amplicon


Confirmation sequences for line 138B08 and hit At5g07660 are shown.
Line ID 138B08
Locus At5g07660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2429870
Primers used for PCR 09kk - 8474, show primer details
   
Sequence Name 67-K113595-0022-138-B08C-09kk
GenBank Accession LN511856 [GenBank]
Sequencing primer gene specific primer, 09kk: 5'-TGTTTATGGTAATCTCCTTTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113595-0022-138-B08C-09kk
TATCATACCCACTTCTTCTCATAACTTGGAGCTTCTAAAACAAAATTCAAACACCTTGAG
AGAAATAACGTGTTAACGATTCGGTTTCTGCAACCTCAGGACGCAGTTAGCAGGAAAATC
AGCTTAGACACATTGATTGATTTCGCATTGAAACAAGGTTCACAGTGGATGTTCATCACT
CCTCATGATATAAGGTAACTTCCATCCACTTAGTGAAGTAACACACAACACTGTTTCTTT
AAAGCATAGGAACTATGTAACATTGTAATCTCTCTTTTGCAAATTACACAGTATGGTGAA
GTCACACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 25-K113594-0022-138-B08C-8409
GenBank Accession LN497530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113594-0022-138-B08C-8409
TCATTGAATATATCCTGTGTGACTTCACCATACTGTGTAATTTGCAAAAGAGAGATTACA
ATGTTACATAGTTCCTATGCTTTAAAGAAACAGTGTTGTGTGTTACTTCACTAAGTGGAT
GGAAGTTACCTTATATCATGAGGAGTGATGAACATCCACTGTGAACCTTGTTTCAATGCG
AAATCAATCAATGTGTCTAAGCTGATTTTCCTGCTAACTGCGTCCTGAGGTTGCAGAAAC
CGAATCGTTAACACGTTATTTCTCTCAAGGTGTTTGAATTTTGTTTTAGAAGCTCCAAGT
TATGAGAAGAAGTGGTGTATGATATTATACTATGCAAACCGAGATCAAGTTGTAAGAAAT
AAAATTCCCAAAAGGAGATTANCATAAAC

Last Updated on 06.12.2018 16:11