Genotyping and primer details


Genotyping and primer details for 138B08 - At5g07660
Line ID 138B08
Locus At5g07660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2429870 of line 138B08 Chr5:2429870
   
Insertion allele amplicon 8474 - 09kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09kk: 5'-TGTTTATGGTAATCTCCTTTTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2429497
   
wt amplicon (over InsPos) 02lb - 09kk
wt amplicon start Chr5:2429497
wt amplicon end Chr5:2430421
wt amplicon size 925 bp
Locus specific primer 09kk: 5'-TGTTTATGGTAATCTCCTTTTGGG-3'
2nd locus specific primer 02lb: 5'-TGATACTTCACATTCTCCGTCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11