Sequences from confirmation amplicon


Confirmation sequences for line 138D02 and hit At5g64070 are shown.
Line ID 138D02
Locus At5g64070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25639380
Primers used for PCR CL25 - 3144, show primer details
   
Sequence Name 41-K028748-022-138-D02C-3144
GenBank Accession LN503954 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K028748-022-138-D02C-3144
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTTCGGTTTAAACTATCAGCGTTTGAT
AGTGAGAAATGAGTCGCACAGCAAGATGTATTGCGAGAAATGAGTTGCACAGCAAGATGC
TCCTGCCTACAGTCGTCACCGCTCTTCACAATGATCTGCAAAAATCTAGAGTTATTTAAT
AATAGAGGTAGAAAAGAATATCATATGGCATAATTGTCAACACACAAGGACACAATTCAA
TAAAGATTAAACACGGAAAGTATCTAACATCTTTAGTAACAGCTTTTTAATGACAATACA
AATTTGGCAGGACATACATAAGCAAAGTCTACATTATTGCAGGCTGGGAAGAGAAAAATG
CATGATCAAGATCGAAGTAGTGTCATATAGTTCATAGTTACTTTTCAATTATCGGGTAAA
AATCGAGCTCACCCTAGACCTTAATATTCCTTAATAAACATTCTGGAAAGTCTGCAGACA
AGGA
   
Sequence Name 41-K028748-022-138-D02C-CL25
GenBank Accession LN503955 [GenBank]
Sequencing primer gene specific primer, CL25: 5'-TGATACCGCTAATCTTCCTTGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K028748-022-138-D02C-CL25
AGGTCTAGGGTGAGCTCGATTTTTACCCGATAATTGAAAAGTAACTATGAACTATATGAC
ACTACTTCGATCTTGATCATGCATTTTTCTCTTCCCAGCCTGCAATAATGTAGACTTTGC
TTATGTATGTCCTGCCAAATTTGTATTGTCATTAAAAAGCTGTTACTAAAGATGTTAGAT
ACTTTCCGTGTTTAATCTTTATTGAATTGTGTCCTTGTGTGTTGACAATTATGCCATATG
ATATTCTTTTCTACCTCTATTATTAAATAACTCTAGATTTTTGCAGATCATTGTGAAGAG
CGGTGACGACTGTAGGCAGGAGCATCTTGCTGTGCAACTCATTTCTCGCAATACATCTTG
CTGTGCAACTCATTTCTCACTATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGAC
AATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA
GGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACAT
CAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTG
GGTGG

Last Updated on 06.12.2018 16:11