Sequences from confirmation amplicon


Confirmation sequences for line 138E01 and hit At5g03180 are shown.
Line ID 138E01
Locus At5g03180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:754511
Primers used for PCR 12lm - 8474, show primer details
   
Sequence Name 57-K114129-0022-138-E01C-12lm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12lm: 5'-ATTTTATGTGTCATGTGTGTGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114129-0022-138-E01C-12lm
AAACCCAATTCTAATCCCACCCTAACCCCTTTAATAAAGCCTAACCCAAAACCCAATTCC
CAACCGGTAATTTGAAACCCAACCCACCCCTTTAGGAACTCTACCTTCCCCCAAAAAATC
TCCCCGCTTGTCAAACCCCCTAATAATTTGCGGGCTCTTCGGGAAGGGGGAATTTGCTTC
CCCCCCTTTTCAAATACCCTTAAAAACCTGTTGTCCCGGCCTGGGGGATCCCAATCCCAA
TCATATGGCTTAAAACAATGCACTTTACCGGTTGCTTCCCCTACCCCCAATTAATTGAAA
AAAAGTGTAAGGAATCAAAACCCCTTCAACAAATTAGGGTTTCCCTGTAACACCAAATAA
GGGTTTCTCTGTAACTTTACTTCAATTCTTACACCTCTGTAAAAAACCTAACCAAAAAGG
CCCCCAAACCCAAATTGACCAACAATTTGTAAAGGGTTCCTTGCCCGGAAATTCTCCTGG
GATCACCATTGATTATAAGGGTCAATTTGGAAAAAAAAAAAAATTATTTCCCATTTTTTT
TCCATTCCAAAATTGTAAATTCCCCCACCTTTATA
   
Sequence Name 15-K114128-0022-138-E01C-8409
GenBank Accession LN493062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114128-0022-138-E01C-8409
GAGCCTTTACATTTGTCGTTCAGTTCGGTTTTGTGGTCCTTTTCGCTCAGCTCTTCTACA
GAGTTGTAAGATTTGATGTAATGTCACAGAGAAACCTTACTTTGTTGTCACAGAGAAACC
TTACTTTGTTGAAGAGTTTTTGATTCCTCACACTCTCTCTCATTAACTTGTGTGTAGGTG
AAGCAGCCGGTAATGTGCATTGTCTTAGCCACTATGATCGGATTTGGACTCACCATGACC
GGCACAACAGCTATTAACGAGTATTTGAAATGGAGGAGAAGCAATTCCCACCTGCCAGAA
GAGCCAGCAAGTACTCAGGTGGTTTGACAGCAGCGTAGATCTTTTGAGTGAAGCTAGAGT
CCCTAAAGGGTTGGATCGGTTTTCAATTAACCGGTCGGGATTCGGTTTTCGGTTTAGCTT
TAATCGACTTGTCTAGGTTGAGATCAGATTTGGTTTTCAATACTTCCAAGTCTTTTTTTT
TTTCCCACCTAAAATATAGGGAATGATGATGGGCCCCCCCTTGACCCTTAAA

Last Updated on 06.12.2018 16:11