Sequences from confirmation amplicon


Confirmation sequences for line 139D12 and hit At5g57200 are shown.
Line ID 139D12
Locus At5g57200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23178354
Primers used for PCR 05wt - 8474, show primer details
   
Sequence Name 29-K118761-0022-139-D12C-05wt
GenBank Accession LN499495 [GenBank]
Sequencing primer gene specific primer, 05wt: 5'-CAACATCCTTGTTCTATGCGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118761-0022-139-D12C-05wt
GTTCTGTTTCTTCATGATGAGAAGTTAGGAGACAAACNCTTTGAAGCTGCACCTGAAGCT
TTTGGTAAACGCTCTGCCTCGATGTCATACTTTAAAACCCGATAACATTCAANGCCGCTC
TTCTAGAAAGAGCGCGTATGTTCTAACCCAAGCAGAACAATCCCACGCTGCATCAAGAAA
CAAATATATGAATTGGTCAAGTTTACAAGAACTGAAAACATTTGCATATATCAAGATAAA
GAATTAGTTTATTCACCAAGAGGACTTGTGTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATTAT
   
Sequence Name 04-K118760-0022-139-D12C-8409
GenBank Accession LN488124 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118760-0022-139-D12C-8409
CATTTACAATTGACACAAGTCCTCTTGGTGAATAAACTAATTCTTTATCTTGATATATGC
AAATGTTTTCAGTTCTTGTAAACTTGACCAATTCATATATTTGTTTCTTGATGCAGCGTG
GGATTGTTCTGCTTGGGTTAGAACATACGCGCTCTTTCTAGAAGAGCGGCTTGAATGTTA
TCGGGTTTTAAAGTATGACATCGAGGCAGAGCGTTTACCAAAAGCTTCAGGTGCAGCTTC
AAAGGTTTGTCTCCTAACTTCTCATCATGAAGAAACAGAACACATTTTCAGAAACTTTTG
TTCAATATGGTTTTTGTAGACGCATAGAACAAGGATGTTG

Last Updated on 06.12.2018 16:11