Sequences from confirmation amplicon


Confirmation sequences for line 140B11 and hit At2g36590 are shown.
Line ID 140B11
Locus At2g36590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15343389
Primers used for PCR RK16 - 8474, show primer details
   
Sequence Name 53-K015973-022-140-B11C-8409
GenBank Accession LN507980 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K015973-022-140-B11C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATGTACCCGCCTCTTGCCATGATCCT
AAACAACAAGTTCTTTAATGCCAATGGGTTTCCTTTGATCCCAAACTTGGTGTCCATGTA
CTCATATGTTGGACTTGCAAAAATCTACAAAAATTGAGTAAGAACGGATTGACTAACACA
TAATTCTTTTCTCGAAGAGAAACTTACGTGCAACGAGATAACGGATTGAAGGATAGCGGA
GATGTTAGCAAGGGCTTTAACCCAAAGTGGACCATTAACGTTGTTTAGTAGATAGGGCGA
GGTCGAGGATCCGTAAGCCCAATACCCGATGAATACAACCGCAAACATCGGTAAAACACC
CACCGTGAATTGAAAGTATAGAGCCTTCATCATGTTTTTTACTACTGGTTGCTTTACTGT
TGCCTATCAATATCATGACGGAAAAAGCAGTGTAGATTAGAAGGATTGTCAAGCAGTGTA
GTAAAATACACACAC
   
Sequence Name 54-K015973-022-140-B11C-RK16
GenBank Accession LN508319 [GenBank]
Sequencing primer gene specific primer, RK16: 5'-AGGCTTCAAAGGTTTAGTGTGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K015973-022-140-B11C-rk16
CTACACTGCTTGACAATCCTTCTAATCTACACTGCTTTTTCCGTCATGATATTGATAGGC
AACAGTAAAGCAACCAGTAGTAAAAAACATGATGAAGGCTCTATACTTTCAATTCACGGT
GGGTGTTTTACCGATGTTTGCGGTTGTATTCATCGGGTATTGGGCTTACGGATCCTCGAC
CTCGCCCTATCTACTAAACAACGTTAATGGTCCACTTTGGGTTAAAGCCCTTGCTAACAT
CTCCGCTATCCTTCAATCCGTTATCTCGTTGCACGTAAGTTTCTCTTCGAGAAAAGAATT
ATGTGTTAGTCAATCCGTTCTTACTCAATTTTTGTAGATTTTTGCAAGTCCAACATATGA
GTACATGGACACCAAGTTTGGGATCAAAGGAAACCCATTGGCATTAAAGAACTTGTTGTT
TAGGATCATGGCAAGAGGCGGGTACATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11