Sequences from confirmation amplicon


Confirmation sequences for line 140F01 and hit At1g47271 are shown.
Line ID 140F01
Locus At1g47271 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17329324
Primers used for PCR 09oc - 8474, show primer details
   
Sequence Name 28-K114720-0022-140-F01C-09oc
GenBank Accession LN498964 [GenBank]
Sequencing primer gene specific primer, 09oc: 5'-CAAAACCCAATCTCTGTTTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114720-0022-140-F01C-09oc
CTTTCGTGAGAGGACTATTAGCAAGAGCTAATGAGGTCCCAAAGATTTCATCAGAGGAAG
GTATCGGCCGGTTTTGGTTGCTTAACTTCGCCTCCGCCGCCGGAGAAAGGGTTGGAGAAT
CTGACGGTGGCGGATGTGCTCTCCACGAAGGATACTGACATTGATACGTGGATCTCGTGC
CGTACGAATGGACACTGTTTCTGACGCTGTTAAAAATGTTCGTTCAAACTTCTTTCTTTT
TCTCCGTTTTTATTGTTTGCTCATCACGTTACACACGCATCATTTAGTACCCATGAAAAC
GAGATTTTTAAAACAATGTATCAATTTTATGGCATTAGTTAGCTTAATATGTTTTTGGGG
TACAATTCTTTACCTTTGGACATTTAAGCTGCCATAAGTCTATAAATCTCGAACTAAAAA
CTCATACCAGAACTATTTTATTTACTTATTAATGTTAGGTTCGTTGCAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCANAAATGTAGA
   
Sequence Name 08-K114719-0022-140-F01C-8409
GenBank Accession LN489840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114719-0022-140-F01C-8409
CACGAACCTAACATTAATAAGTAAATAAAATAGTTCTTGTATGAGTTTTTAGTTCGAGAT
TTATAGACTTATGGCAGCTTAAATGTCCAAAGGTAAAGAATTGTACCCCAAAAACATATT
AAGCTAACTAATGCCATAAAATTGATACATTGTTTTAAAAATCTCGTTTTCATGGGTACT
AATTGATGCGTGTGTAACGTGATGAGCAAACAATAAAAACGGAGAAAAAGAAAGAAGTTT
GAACGAACATTTTTAACAGCGTCAGAAACAGTGTCATTCGTACGGCACGAGATCCACGTA
TCAATGTCAGTATCCTTCGTGGAGAGCACATCCGCCACCGTCAGATTCTCCAACCCTTTC
TCCGGCGGCGGAGGCGAAGTTAAGCAACCAAAACCGGCCGATACCTTCCTCTGATGAATC
TTTGGGACCTCATTAGCTCTTGCTAATAGTCCTCTCACAAAAGCTTTCATTATATAATCT
TATTAGGTTCAAAATTGTACTTTTTGCAAAAATAAATGAAACA

Last Updated on 06.12.2018 16:11