Sequences from confirmation amplicon


Confirmation sequences for line 140G03 and hit At5g66220 are shown.
Line ID 140G03
Locus At5g66220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26461619
Primers used for PCR PH84 - 8474, show primer details
   
Sequence Name 35-K018752-022-140-G03C-PH84
GenBank Accession LN501639 [GenBank]
Sequencing primer gene specific primer, PH84: 5'-CTTCCCTATCATCGACTCAAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K018752-022-140-G03C-PH84
CTCACCCAACAACTTATTCTTGATAACCGCTTTTCCAGTTTTCGGGACTCTCTCCTCTTT
CGAAAATGCAATCTGCATCACAAGTTGTCAAAAGATCTCGATCATTCATTCGACCAATAT
TATTCGAAATGATAATCCGAAAAATAAAGTTGACTCACTGTGAGAGAGCCTTTAGGACAG
ATAGCGAAGATGATTGAAGAGCCGGGAGGGAAATCTTGGTTTTTGAAGACCATCAAGAAT
TGGTCAATGGCTTTGGCTTCGGATCTTGTGTATTTCCCTGACGCTTTCATGATCTCCTCA
CAGTTTTCTACTACTTTCTCCGAGTATTGTATCCCCGTTAACCTCTTTTTCATCGTCACC
CTTGCCAATTTCTCAAACTCACCTTTTCCCACCAGAGAAGAAAATTTAAAAAATGTAAAT
GATTAGATATTCAACTCACATTGAATTATTTTACAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACTTTTTGATAAAACGACAAATT
   
Sequence Name 39-K018752-022-140-G03C-8409
GenBank Accession LN503191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K018752-022-140-G03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAAAATAATTCAATG
TGAGTTGAATATCTAATCATTTACATTTTTTAAATTTTCTTCTCTGGTGGGAAAAGGTGA
GTTTGAGAAATTGGCAAGGGTGACGATGAAAAAGAGGTTAACGGGGATACAATACTCGGA
GAAAGTAGTAGAAAACTGTGAGGAGATCATGAAAGCGTCAGGGAAATACACAAGATCCGA
AGCCAAAGCCATTGACCAATTCTTGATGGTCTTCAAAAACCAAGATTTCCCTCCCGGCTC
TTCAATCATCTTCGCTATCTGTCCTAAAGGCTCTCTCACAGTGAGTCAACTTTATTTTTC
GGATTATCATTTCGAATAATATTGGTCGAATGAATGATCGAGATCTTTTGACAACTTGTG
ATGCAGATTGCATTTTCGAAAGAGGAGAGAGTCCCGAAAACTGGAAAAGCGGTTATCAAG
AATAAGTTGTTGGGTGAGGCAGTTCTTGAGTC

Last Updated on 06.12.2018 16:11