Sequences from confirmation amplicon


Confirmation sequences for line 141C11 and hit At1g70730 are shown.
Line ID 141C11
Locus At1g70730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26670666
Primers used for PCR 05ai - 8474, show primer details
   
Sequence Name 41-K106635-0022-141-C11C-8409
GenBank Accession LN504090 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K106635-0022-141-C11C-8409
GCCTTTACATTGAATAATCCTGTGGCGAGCCTCCAGAGTTTGGCGCCGCTGCTGATGGTG
ATGCAGACCGAAACATGATCCTCGGTAAAAGGTAGCAGAGACTAATTCTCTTTTAAGTGA
GATCACAGAGATGTGGAGCGGATATACCACTCTCCCCGTTAACCAAAAATATCTCTTCTC
CGCAGGTTTTTCGTAACTCCTTCGGATTCAGTTGCTATAATTGCTGCAAATGCTGTTGGG
GCCATACCATACTTCAGCTCTGGTTTAAAAGGTGTTGCTAGGTATGCATCCCGGTTCTTC
TTTGTTTACTCCAGTTCATAGATTGAAAACGCTGGACTTTGGTGTCATACGTTTTTGTTT
ATTATAGATAGTAACAATCTTTTCTCATTAAATTCTCAACTTTCACATTCGTATTGCAGG
AGCATGCCGACCTCAGCTGCACTTGATGTCGTTGCAAAAAACTTGGGTTTGAAGTTCTTT
GAGGTAGATGTCATTGTGAAGCTTTTCTCTAGCTTCTATTCATTGCAATCACGT
   
Sequence Name 57-K106636-0022-141-C11C-05ai
GenBank Accession LN509429 [GenBank]
Sequencing primer gene specific primer, 05ai: 5'-TACGTGATTGCAATGAATAGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K106636-0022-141-C11C-05ai
CTACCTCAAGAACTTCAACCCAAGTTTTTTGCAACGACATCAAGTGCAGCTGAGGTCGGC
ATGCTCCTGCAATACGAATGTGAAAGTTGAGAATTTAATGAGAAAAGATTGTTACTATCT
ATAATAAACAAAAACGTATGACACCAAAGTCCAGCGTTTTCAATCTATGAACTGGAGTAA
ACAAAGAAGAACCGGGATGCATACCTAGCAACACCTTTTAAACCAGAGCTGAAGTATGGT
ATGGCCCCAACAGCATTTGCAGCAATTATAGCAACTGAATCCGAAGGAGTTACGAAAAAC
CTGCGGAGAAGAGATATTTTTGGTTAACGGGGAGAGTGGTATATCCGCTCCACATCTCTG
TGATCTCACTTAAAAGAGAATTAGTCTCTGCTACCTTTTACCGAGGATCATGTTTCGGTC
TGCATCACCATCAGCAGCGGCGCCAAACTCTGGAGGCTCGCCACAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR 25uh - 8474, show primer details
   
Sequence Name 21-K117104-0022-141-C11C-25uh
GenBank Accession LN495830 [GenBank]
Sequencing primer gene specific primer, 25uh: 5'-TCTCTCGTCTCTACTTCGCCTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117104-0022-141-C11C-25uh
GTTCGCTTTCGCTTCTTCTCCTTGTTATATCAATCCCCGCGTTCTTATTTCGAAATTGGA
TTAGAGATTTGAATTCTAAATTCAGATCTTCGCAACGAAATTGATTTCTTCATTTAGTTC
CGATCTTATGGATTGAATGAATTTTGTGTGTTGGTTTTAACAATTTGATCCGATCCGATG
AGATTTTATGGACAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAG
   
Sequence Name 05-K117103-0022-141-C11C-8409
GenBank Accession LN488522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117103-0022-141-C11C-8409
AAATCTCATCGGATCGGATCAAATTGTTAAAACCAACACACAAAATTCATTCAATCCATA
AGATCGGAACTAAATGAAGAAATCAATTTCGTTGCGAAGATCTGAATTTAGAATTCAAAT
CTCTAATCCAATTTCGAAATAAGAACGCGGGGATTGATATAACAAGGAGAAGAAGCGAAA
GCGAACGAACCTTCTTACGGAGTCCAGAAGTCCCAGGTTTCTGGCCATCAATAGGCGAAG
TAAGNACGAGAGA

Last Updated on 06.12.2018 16:11