Sequences from confirmation amplicon


Confirmation sequences for line 142G07 and hit At5g40760 are shown.
Line ID 142G07
Locus At5g40760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16313735
Primers used for PCR ms32 - 8474, show primer details
   
Sequence Name 03-K024389-022-142-G07C-8409
GenBank Accession LN487317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024389-022-142-G07C-8409
GTATATTTAAATTCAAAAAGCTAAAAGAAGATTCAAAACACGATGTCAGGGCAAGACAAT
ACATCTGAATATGTCGCCTGGGACATCCTTGAATTGAATGCGAATTTCTGCCTTCCTTGA
ATTCAACGCTTTTCCGGCCTTTAATATAAATGGAACACCTGAAAGATATATCCAGGACCG
TTAGAATGTGCATGCAAACATGTCGGATTTTGGTTCCACATGCTTTAACCAAAAGATTGA
TGCTATCTATGATCATGTTGACCCATCAAACCTCTGTTTTTTCTTCATATTTTATGTCAA
TTTCCAGATTGTTTCTGAACTGATAGATGAAAATATGAAGAGTGAAGTGGGACATGGTAC
CTTCCCATCTTTCGTTGTGTATGCGAAGGATTGTTGTGGCAAATGTTGGAGTGTTTGAGT
CGTTTGGAACAGTATCATCATCCCTATATCCTTCGTATTGTCCAAGAACTACCTCGTCAT
CGCTTATCGGAACCACAGATTGAAGCACCTGAAGTTCACTAAAATAATCAGTAACGAGAG
ACTAATTGGCTGAAAATGCAAATGGAAATATCAACCAATCCATGTAAACTCGCTAAGGAG
ATTTTTAAAGAAAAATGTAACTAGATCAAACAGTGAGGTTCTCAATCATATGAATTCAGA
TAAAAGAGGCAACGAANACCAGATAGAATGGCAAAAAGTGGTCAAGAAAAGTATTTGTTC
ACCTTCACTTTCTCATCCCGGATATGCT
   
Sequence Name 04-K024389-022-142-G07C-ms32
GenBank Accession LN487756 [GenBank]
Sequencing primer gene specific primer, ms32: 5'-GAGATGACACCAGTTCATTTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024389-022-142-G07C-ms32
CATCTTCACTTCTTGCCACACAGAATAATCCGTGATATTATTCAAAACCATCTGCTACAG
GCAAGTATACCTGAGATACTTTTCCTGATAACAAAACTTCATTGGAAATCTATGTGATTG
TTTTTCAGAGTCTCTCTGTAAGTTCAAATGCTTTCATGTATTGACTAACTGAGTTCATAA
TACTCTGTAGGTTCTTTGCCTTGTTGCCATGGAGAAACCAATATCTCTTAAACCTGAGCA
TATCCGGGATGAGAAAGTGAAGGTGAACAAATACTTTTCTTGACCACTTTTTGCCATTCT
ATCTGGTTTTCGTTGCCTCTTTTATCTGAATTCATATGATTGAGAACCTCACTGTTTGAT
CTAGTTACATTTTTCTTTAAAAATCTCCTTAGCGAGTTTACATGGATTGGTTGATATTTC
CATTTGCATTTTCAGCCAATTAGTCTCTCGTTACTGATTATTTTAGTGAACTTCAGGTGC
TTCAATCTGTGGTTCCGATAAGCGATGACGAGGTAGTTCTTGGACAATACGAAGGATATA
GGGATGATGATACTGTTCCAAACGACTCAAACACTCCAACATTTGCCACAACAATCCTTC
GCATACACAACGAAAGATGGGAAGGTACCATGTCCCACTTCACTCTTCATATTTTCATCT
ATCAGTTCAGAAACAATCTGGAAATTGACATAAAATATGAAGAAAAAACAGAGGTTTGAT
GGGTCAACATGATCATAGATAGCATCAATCTTTTGGTTAAAGCATGTGGAACCAAAATCC
GACATGTTTGCATGCACATTCTAACGGTCCTGGATATATCTTTCAGGTGTTCCATTTAT

Last Updated on 06.12.2018 16:11