Sequences from confirmation amplicon


Confirmation sequences for line 142G09 and hit At3g47550 are shown.
Line ID 142G09
Locus At3g47550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17524902
Primers used for PCR CD25 - 8474, show primer details
   
Sequence Name 05-K104193-0022-142-G09C-CD25
GenBank Accession LN488332 [GenBank]
Sequencing primer gene specific primer, CD25: 5'-TATCAACATGGATACACTGCACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104193-0022-142-G09C-CD25
CTATATTCACATCGGGTAATTTGCTTAACATAGAGAGTTATGACTTGTTGAATTGTTGTA
CAAGTGGATTATCTGTAACCAGTTATAACAAAATTAATAATACCTTGTGAAGAACTTATC
AAGTTAACAAAGTAGTGAGTGACCTGTGTTGTGTGCAGTGACGATTGGGAGAACGGAGTT
CCGTTGGACTTGACCGACCCGCGCATTCTGGCAATGGCGGCTGCAGAACGTCATTTCTTG
GAAGCAGACTATGATGAATATTCTGAGAATAACTCAAGCGGAGCTGCCTTTTGTCGCTCT
GCTGCTCTCATCGTAAGCTCTCAGTTGAGAAGATGTTTTCTTACCATGATTTAATACTAA
ATGCCTGCACTCTAACTTCTGTAATGGCAGCTAATGGCACTTTTACTGTTACGAGATGCG
CTAAATCTCACAACGAACCCAGACGACGAGGACGATCCAACTGCCTTCTTCTCTGTATGT
TCTATGTTCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTACCCATTTTTTTTC
CATTTCAAAATGTAGATGTCCGC
   
Sequence Name 21-K104194-0022-142-G09C-8409
GenBank Accession LN495638 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104194-0022-142-G09C-8409
ATTTACATTGAATATATCCTGCGAACATAGAACATACAGAGAAGAAGGCAGTTGGATCGT
CCTCGTCGTCTGGGTTCGTTGTGAGATTTAGCGCATCTCGTAACAGTAAAAGTGCCATTA
GCTGCCATTACAGAAGTTAGAGTGCAGGCATTTAGTATTAAATCATGGTAAGAAAACATC
TTCTCAACTGAGAGCTTACGATGAGAGCAGCAGAGCGACAAAAGGCAGCTCCGCTTGAGT
TATTCTCAGAATATTCATCATAGTCTGCTTCCAAGAAATGACGTTCTGCAGCCGCCATTG
CCAGAATGCGCGGGTCGGTCAAGTCCAACGGAACTCCGTTCTCCCAATCGTCACTGCACA
CAACACAGGTCACTCACTACTTTGTTAACTTGATAAGTTCTTCACAAGGTATTATTAATT
TTGTTATAACTGGTTACAGATAATCCACTTGTACAACAATTCAACAAGTCATAACTCTCT
ATGTTTAAGCAAATTTACCCGATGTGAATTATAGTTTCATCAGGAGGAGGAGGCGGAGGT
GCAGTGT

Last Updated on 06.12.2018 16:11