Sequences from confirmation amplicon


Confirmation sequences for line 142H04 and hit At3g61740 are shown.
Line ID 142H04
Locus At3g61740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22851262
Primers used for PCR 01do - 8474, show primer details
   
Sequence Name 30-K109936-0022-142-H04C-01do
GenBank Accession LN499762 [GenBank]
Sequencing primer gene specific primer, 01do: 5'-ACTTTAGGTTGTTGGCTGTGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109936-0022-142-H04C-01do
AAATTGCTACGCCGGANAAGTATCTAGCTGAATGTACATTTAATGAATCAATCGGAAAGA
AGCCAGCATCATGTCAAGGGATTTCCCGTAAGCTAGCCGCCGAGGAAACAAATCGGTTAG
CTTCCCACCGCCATTACTGTTCAATTAGAAAGCAAGTTTGAACGTTTGCAAGAATGGTTT
AGACTTAAGAGAGCTCTAGTTCATGAATTTGCGGCAGTTTGGAGCTTTACAAAGGCAGGG
CACTTTGATCTCTTCAGATTCATCAACCTCAAAGAGATAATCATACCTGTAGAGAAATAG
GTTTTCATGATCAAGGTTAATAATGGATCAGCAATGAGTATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K109935-0022-142-H04C-8409
GenBank Accession LN492553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109935-0022-142-H04C-8409
ATTGAATACTCATTGCTGATCCATTATTAACCTTGATCATGAAAACCTATTTCTCTACAG
GTATGATTATCTCTTTGAGGTTGATGAATCTGAAGAGATCAAAGTGCCCTGCCTTTGTAA
AGCTCCAAACTGCCGCAAATTCATGAACTAGAGCTCTCTTAAGTCTAAACCATTCTTGCA
AACGTTCAAACTTGCTTTCTAATTGAACAGTAATGGCGGTGGGAAGCTAACCGATTTGTT
TCCTCGGCGGCTAGCTTACGGGAAATCCCTTGACATGATGCTGGCTTCTTTCCGATTGAT
TCATTAAATGTACATTCAGCTAGATACTTTTCCGGCGTAGCAATTTTGTAAATGTCTAGA
ATTCACCGACTCTAAGAAAATAACTTCACAGCCAACAACCTAAAG

Last Updated on 06.12.2018 16:11