Sequences from confirmation amplicon


Confirmation sequences for line 143C07 and hit At5g47075 are shown.
Line ID 143C07
Locus At5g47075 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19122535
Primers used for PCR 02xq - 8474, show primer details
   
Sequence Name 49-K119512-0022-143-C07C-02xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02xq: 5'-GTATTTGATATTCAATAGGCCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K119512-0022-143-C07C-02xq
AAGTTTATTTTTTTTTTCATAAATTTACATCATCATCCATATTCAACTTTATCATCATCA
TTAATATTATGTTGTTGTGGTTAACCATCTGAATTATTGACCTACTTTCGTATTAATCAA
TTTTAATTTTGTACTTATCCTTTATCTTTTAAGTATCATTCCACTTATGTATATATGTTA
CTAAAAAACATATGGATACTATCAAAAATATATATGTTACTAAAAACTATCCATATTTTT
TTAGTAACATATATACACAAAGAGAAAAAAATCAAAAATGATAATATATGCATATATATA
TATATATATATATATATATATATGTGTGTGTGTGTCTTTCTATGTGATATTTCTTTATAT
AGCCCCGTTTTTCTCATTATTTTTGTAAACTCCAATTATCATACAATGACTAAACCCCCC
TTTCTTTTTGTCTACATCACCCTTATTCTGGGTTTTTTTAACCCTCTCTAATTCAGATAA
AAAATCGTGTATTTTTACTACCAAGAAAACAAACTGTGTGTGTGTGTTTAAAATATCACT
TTCACGGGATCAGGGGTGAAAGAGATTCAGGGACAAAACGCAATATGTCAAATTTTTTTA
TCAAAAACAGATTGTGGGGCGGGCTCGTGTACCGCGTTGTGCTTGCATTTATGGAAAGGA
ATTGGCATATGTTTTAAAACAGATGATCATAAATTTTTATGCCTCTGCAAATATGATGTC
CGGGAAATCTACGTGGATCACCAATGAGTATGAGGGTCAAT
   
Sequence Name 17-K119511-0022-143-C07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119511-0022-143-C07C-8409
CATATAATTTATGATCATCTGTTTTAATACATTTGCCAATTCCTTTCCATAACTGCAAGC
ACAACGCGGTACACGAGCCAGCACCACAATCAGTTTCCGATAAAAACTTTTGACATATTG
CGTTTTGTCCTTGAATCTCATTCACCATTGATCCTGTAAAAGTAATATTTAAAACACAAA
CACAAAGTTAGTTTTCATGGTAGTAAAACTACATGATTTTATAACTGAATTAGAGATGGT
TAAAAAAACCTAGAATAAGGATGATGTAGACAAAAAGAAAGGTGGATTTAGTCATTGTAT
GATAATTGGAGTTTACAAAAATAATGAGAATAACGGTACTATATAATGAAATATCACATA
GAAAGACACACACACAGATATATATATATATATATATATATATATATATGCATATATTAT
CATTTTTGATTTTTTTCTCTGTGTGTATATATGTCACAAAAAAAATATGGATAGTTTTTA
GAAACATATATATTTTTGATAGTATCCATATGTTTTTTAGTCACATATATACATAAGTGG
AGTGATACTTAAAAGATAAGGGATATGTACAAAATTAAAATTGATTAATACGAAAGTGTG
TCAATAATTCAGATGGTTACCCACAACAACATAATATTAGTGATGATGATAAAGTTGAAT
ATGGATGATGATGTATATTTATGAAAAAAAAAATAAACTTTTATATATATACACTTTGGT
TAAAAAATTTCCCGGCCT

Last Updated on 06.12.2018 16:11