Sequences from confirmation amplicon


Confirmation sequences for line 143D03 and hit At5g23070 are shown.
Line ID 143D03
Locus At5g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7742813
Primers used for PCR KB67 - 3144, show primer details
   
Sequence Name 18-K100851-0022-143-D03C-3144
GenBank Accession LN494222 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K100851-0022-143-D03C-3144
ATCCCCTATCTTCACAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCT
TGAATCCAAAACGGCACGAGCGGTTTCCAAAACGTTTTGACCGCAAACGTAATGACTCCT
ACACACAGGCATATAAACTTCAGCACCACCGATCAACTCCGTCTCTTTCTCCTCCGTCTT
CCTCATCGTAAACAAAGCTCTCTTCCCACAAACCTCACACCGTGACGTCAGCTTCGTAAC
CGTATCCGCAATCGGAATCAAATCAAGAACCGAACCAAACCTCCTCCTCATAAAATCACC
ATCCAATCCAGCAACAATTACAGTTTTACCCTCTTTATCAGCAGCTTCACGGCAAAACTC
GTAAAGATCTCCGAAGAATTGAGCTTCGTCGATTCCAATCACATCTAATCGATTCTCGTA
GTCGTCGAATCCGAATCTCTCTTTGAAGGACGAGAGATCGGGGAGTGACCAGCAAGGGTA
TTTCTCACCGTCGTGAGTAACTATTGATTCGGTGCAGTATCTTGTGTCTTTGTTGGATTT
GATGATTGCGATTCTTTTACCGGTTTCTCTTTCGGCGAGTATACGGCGGAGAAGTGTTGT
TGTTT
   
Sequence Name 18-K100857-0022-143-D03C-KB67
GenBank Accession LN494223 [GenBank]
Sequencing primer gene specific primer, KB67: 5'-AATGTTCTCCGGTAAAACAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K100857-0022-143-D03C-KB67
GAAGAGAACCGGTAAAGAATCGCAATCATCAAATCCAACAAAGACACAAGATACTGCACC
GAATCAATAGTTACTCACGACGGTGAGAAATACCCTTGCTGGTCACTCCCCGATCTCTCG
TCCTTCAAAGAGAGATTCGGATTCGACGACTACGAGAATCGATTAGATGTGATTGGAATC
GACGAAGCTCAATTCTTCGGAGATCTTTACGAGTTTTGCCGTGAAGCTGCTGATAAAGAG
GGTAAAACTGTAATTGTTGCTGGATTGGATGGTGATTTTATGAGGAGGAGGTTTGGTTCG
GTTCTTGATTTGATTCCGATTGCGGATACGGTTACGAAGCTGACGTCACGGTGTGAGGTT
TGTGGGAAGAGAGCTTTGTTTACGATGAGGAAGACGGAGGAGAAAGAGACGGAGTTGATC
GGTGGTGCTGAAGTTTATATGCCTGTGTGTAGGAGTCATTACGTTTGCGGTCAAAACGTT
TTGGAAACCGCTCGTGCCGTTTTGGATTCAAGCAAACACTGATGGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCGAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
ATATCA
Primers used for PCR FK11 - 8474, show primer details
   
Sequence Name 40-K101269-0022-143-D03C-8409
GenBank Accession LN503647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101269-0022-143-D03C-8409
GCCTTTACATTGAATAATCAATTTACTATATCAATATACTCATTGCAAGTTCACTTTAGT
TTGTAGGATTTGAGAGTGTTTAGATATTTGGTTTATTAGCTTTGAGATATTTGGAATTTT
AAACATAATTATGTACTATTTAATCAATGATTTTAAGATAACACTCTTATTTGTGAATTT
CTTGTAGAAACCCTTATATCGAAAAAATTTCAAACGCATAATCTAATTTGTTACCAATAT
ATAAATGAAGGACATGACAGTATCTCTCTACCTCAGACGAAGCAATGAACCCAACAACAA
GCTCATCATTTGCTATCTAACCTGACCATCTCGAGACAGTGCAGAGCATCATTCATGGCA
TACCCCAAAACTACGTTATTTCAATGTTCACACAAAAATCTATGCAAGTGCTCTGATTTC
TCTCCAGTTTTAGTCAGTCAACAGCTTTGATTAAGTTTCAGTCTCGTCGCCTCTTCTTCT
CCCGCCTTGACTCTTTTTGTTCTTCGTCTGAGCTATACCGTGAAGAAGAAGACTTCTTGG
TCCTGCGATGCTTTGAGTGCTTCTTGTGGCTACATCTC
   
Sequence Name 40-K101275-0022-143-D03C-FK11
GenBank Accession LN503648 [GenBank]
Sequencing primer gene specific primer, FK11: 5'-AAGAGAAGTAGCCACAAGAAGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101275-0022-143-D03C-FK11
TCTTCTTCCGGTATAGCTCAGACGAAGAACAAAAAGAGTCAAGGCGGGAGAAGAAGAGGC
GACGAGACTGAAACTTAATCAAAGCTGTTGACTGACTAAAACTGGAGAGAAATCAGAGCA
CTTGCATAGATTTTTGTGTGAACATTGAAATAACGTAGTTTTGGGGTATGCCATGAATGA
TGCTCTGCACTGTCTCGAGATGGTCAGGTTAGATAGCAAATGATGAGCTTGTTGTTGGGT
TCATTGCTTCGTCTGAGGTAGAGAGATACTGTCATGTCCTTCATTTATATATTGGTAACA
AATTAGATTATGCGTTTGAAATTTTTTCGATATAAGGGTTTCTACAAGAAATTCACAAAT
AAGAGTGTTATCTTAAAATCATTGATTAAATAGTACATAATTATGTTTAAAATTCCAAAT
ATCTCAAAGCTAATAAACCAAATATCTAAACACTCTCAAATCCTACAAACTAAAGTGAAC
TTGCAATGAGTATATTGATATAGTAAATTGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11