Sequences from confirmation amplicon


Confirmation sequences for line 143G09 and hit At1g25380 are shown.
Line ID 143G09
Locus At1g25380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8904740
Primers used for PCR NR24 - 8474, show primer details
   
Sequence Name 43-K036417-022-143-G09C-NR24
GenBank Accession LN504757 [GenBank]
Sequencing primer gene specific primer, NR24: 5'-AAGGAAGAAGGTTATAGGGGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K036417-022-143-G09C-NR24
ATCATAGCTCTCCTTCCAATTGGGCTGTAAGCACCACTGTTCTCTACAGAGCTTTGTTTC
TTCAAGTGAATTTGTCTTGTCATAAAAGATATTACTTGCCCAACATTCTCCTATGGATCT
TCTATAGGAGATATTTTATGTCTTCTAATCGATTTTAATCTGTTTTTGAATAACTTTAGT
TCCTTCCTTAATCGTTATTCTGGTATCACGCTCAGGTCTACTTCTCAGTTTATGGGAAGC
TAAAGGATGTGCTGCAGTCAAGTGGTTGGTTTTCCTTTGTTACAAATCTTTTGTGCGTTG
TAGCTTATGCTTAACCGGAAGAGTATCATATATATATGCTCAAATAACGTATACCAAGTC
TTTTTTATCAACTGTTCATGTTTGACTGATAGATGGCAAGCTTAGTATTGGGTCAAACAT
GATAGCTGCAGCTGGTGCTGGAGCTGCCACATCTATTGCAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG
   
Sequence Name 35-K036417-022-143-G09C-8409
GenBank Accession LN501739 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K036417-022-143-G09C-8409
GAGCCATTTGCAATAGATGTGGCAGCTCCAGCACCAGCTGCAGCTATCATGTTTGACCCA
ATACTAAGCTTGCCATCTATCAGTCAAACATGAACAGTTGATAAAAAAGACTTGGTATAC
GTTATTTGAGCATATATATATGATACTCTTCCGGTTAAGCATAAGCTACAACGCACAAAA
GATTTGTAACAAAGGAAAACCAACCACTTGACTGCAGCACATCCTTTAGCTTCCCATAAA
CTGAGAAGTAGACCTGAGCGTGATACCAGAATAACGATTAAGGAAGGAACTAAAGTTATT
CAAAAACAGATTAAAATCGATTAGAAGACATAAAATATCTCCTATAGAAGATCCATAGGA
GAATGTTGGGCAAGTAATATCTTTTATGACAAGACAAATTCACTTGAAGAAACAAAGCTC
TGTAGAGAACAGTGGTGCTTACAGCCCAATTTGGAAGGAGAGCTATGATGGTTGGTGAAA
GCCCTCGATACATTCCCCTAT

Last Updated on 06.12.2018 16:11