Sequences from confirmation amplicon


Confirmation sequences for line 144F03 and hit At3g48060 are shown.
Line ID 144F03
Locus At3g48060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17746865
Primers used for PCR 41un - 3144, show primer details
   
Sequence Name 08-K117178-0022-144-F03C-41un
GenBank Accession LN489905 [GenBank]
Sequencing primer gene specific primer, 41un: 5'-TCTCCAGTTATTGTCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117178-0022-144-F03C-41un
GTGCAGGGTCCTCCAAGAGCCTCCACTCACACTTTGAGCAGGACTTCGACCCCGCGGCAG
CTTCACAATCAATTTATTACCGGAACCCTCAGTCAGAGGAACCTCAAGAGTCTTTTCAGA
TGTCAAACTTGATTGAGAAATTTTCTCAGAAGGGACATTCCTGTGTGATGAGAAGCTTCT
ACTCAAACCAGCTCCCCTAGGGGATGCTGACGAAGACGAACCTTGAAACACATTGTTTGA
CTTCCGATGCCTTGAAGAGCCACCGGAACACTTCTTCAAAGTTGAACCAGCAGTAGAGCT
TCTTGCATCTTCTTTTCCACATAAATTGCCTGTTTTAGCATGCTCGCTCGAGCAAGACTG
ACTATTATTGTGCGACTGACTGGAGCTGCTACTTTTCTCATCCTTTACTGCAGCTAGGAC
TTCAGATACACCACCAGCCCCAGCATTTCGCTGCTGTCCATCTTTAGAAACATTACCACC
TGATCCCGGAGAAGGAGCTGATCTTGTGGAGCCTGGCGACGTGGCTACACACTTCAGGTT
GTTTTCCACCTGTTGTTTGACCGAGACAGACTTAGAAGCATGTAGGTGTGATGATGATGT
CTTGTTTGCCTCCGCGGAACCACCTGAATGCCTTCCCCCATGACTCAGTCTTCCAGGCCA
GGAAACACCCTGGTTGGATCCAGACTTCGCATCCATTTCTGCCTCAACTCGTTTCTTCCA
AGTGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTAC
CCTGTCCTCTCCAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGAATGTGCGTCATCCCTTACGTCAGTGG
   
Sequence Name 01-K117177-0022-144-F03C-3144
GenBank Accession LN486750 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117177-0022-144-F03C-3144
CTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTGACACTTGGAAGAA
ACGAGTTGAGGCAGAAATGGATGCGAAGTCTGGATCCAACCAGGGTGTTTCCTGGCCTGG
AAGACTGAGTCATGGGGGAAGGCATTCAGGTGGTTCCGCGGAGGCAAACAAGACATCATC
ATCACACCTACATGCTTCTAAGTCTGTCTCGGTCAAACAACAGGTGGAAAACAACCTGAA
GTGTGTAGCCACGTCGCCAGGCTCCACAAGATCAGCTCCTTCTCCGGGATCAGGTGGTAA
TGTTTCTAAAGATGGACAGCAGCGAAATGCTGGGGCTGGTGGTGTATCTGAAGTCCTAGC
TGCAGTAAAGGATGAGAAAAGTAGCAGCTCCAGTCAGTCGCACAATAATAGTCAGTCTTG
CTCGAGCGAGCATGCTAAAACAGGCAATTTATGTGGAAAAGAAGATGCAAGAAGCTCTAC
TGCTGGTTCAACTTTGAAGAAGTGTTCCGGTGGCTCTTCAAGGCATCGGAAGTCAAACAA
TGTGTTTCAAGGTTCGTCTTCGTCAGCATCCCCTAGGGGAGCTGGTTTGAGTAGAAGCTT
CTCATCACACAGGAATGTCCCTTCTGAGAAAATTTCTCAATCAAGTTTGACATCTGAAAA
GACTCTTGAGGTTCCTCTGACTGAGGGTTCCGGTAATAAATTGATTGTGAAGCTGCCGCG
GGGTCGAAGTCCTGCTCAAAGTGTGAGTGGAGGCTCTTTGGAGGACCCTGCACCAGTGAA
TAGCAGAGTTTCTTCACCTGTGCATACNGTGAAGCANGAACTGTGT

Last Updated on 06.12.2018 16:11