Genotyping and primer details


Genotyping and primer details for 144F03 - At3g48060
Line ID 144F03
Locus At3g48060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17746865 of line 144F03 Chr3:17746865
   
Insertion allele amplicon 3144 - 41un
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 41un: 5'-TCTCCAGTTATTGTCACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17746069
   
wt amplicon (over InsPos) 69vs - 41un
wt amplicon start Chr3:17746069
wt amplicon end Chr3:17747435
wt amplicon size 1367 bp
Locus specific primer 41un: 5'-TCTCCAGTTATTGTCACAC-3'
2nd locus specific primer 69vs: 5'-GCATCCAGAACAACAACTCTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11