Sequences from confirmation amplicon


Confirmation sequences for line 144G09 and hit At1g74140 are shown.
Line ID 144G09
Locus At1g74140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27877054
Primers used for PCR 16tv - 8474, show primer details
   
Sequence Name 19-K116964-0022-144-G09C-16tv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 16tv: 5'-CTACACAGAAATGGCAAAATAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116964-0022-144-G09C-16tv
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 01-K116963-0022-144-G09C-8409
GenBank Accession LN486744 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116963-0022-144-G09C-8409
TCGGATGAGCATTTCATTGATCCTCTTCTCTCATCTCCTTCTTCAGTACCCCACTTTGTT
CCTTCTGCTGTATCGCGATCGGCGAAAGTTCATGGGTTCTTCGCTAGCAAGTTGGGAAAC
ACCAACCTAAAGCTGAAATTTGGAAATGTTATGGAATCTAGAGCTGGGTTCTTCAGTTCT
GAGCTTCCTAGTCATGGATTCGAGTCTGGTGGTTTTACTGGGTTTCAAAAGCGGGGATGG
TATGGAATCGTTTCTTTTCGTCTCTCTTTTTGTGATTTTGCTGGGTTTCAAAGTAAAGTT
CGAAGCTTTCCTAAGAAAAGTTCTGAAATTTCTGAGTTCCTTTTCTCTATGATCCTCTGT
TTCTCGTTAGCTAAGTAAAAGTCTACATTTTTCTGAGATAAACTTGTCTTCCGGATGAAA
TTTCTGGTTTTGATTTAGGGTTTAAACAGAGTTTCTGTGGTAAATTATCGACCTTTTTAA
GCTTTTCTGGGTTAGTACACGTTTGGCTCGT

Last Updated on 06.12.2018 16:11